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Q: I Why does't a negative stain colorize the cells in the smear?
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A student heat-fixes his smear intended for capsule staining. What result might the student expect?
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- Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe how to prepare the media ,, smear from the cheekGive two reasons for heating the slide after the smear is air dried?Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore Stain
- In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?Stain Test. On a piece of white paper, place one or two drops of coconut oil. Note any stained produced on the paper. Place the paper containing the oil drops on a dry evaporating dish and heat for a few minutes in a water bath. Observe what happens. OBSERVATION:What color wouod gram negative cells appear if the decolorizing step was left out of staining procedure? Why?
- what effect would failing to drain the smear in-between staining have on your judgment of the gram reaction of an organism?You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method
- During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture is discarded in the disinfectant solution. Why?Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.Name a few group of microorganisms where Grams staining will not work and/or not appropriate to use. Why?