A student prepares a solution of his dye and measures its absorbance using a 1 cm cuvette. The absorbance is 0.45 and the concentration of the solution is 4.2 * 10-5 M. Determine the molar extinction coefficient at this wavelength (Integer only)
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A student prepares a solution of his dye and measures its absorbance using a 1 cm cuvette. The absorbance is 0.45 and the concentration of the solution is 4.2 * 10-5 M. Determine the molar extinction coefficient at this wavelength (Integer only)
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- Extinction coefficient of a dye is 7200 M-1 cm-1. Assuming a path length of the cuvette to be 1 cm, what is the concentration of solution when the absorbance is 0.300 at 500 nm.Assume a plot of our Abs versus concentration (in M) is linear and has a slope of 7600. Calculate the concentration for the colored species, if the absorbance was 0.360.A 270nm laser is used as excitation of flourescence analysis. A given sample has a flourescence intensity of 1740.0. calculate the concentration of the sample
- The absorbable of a solution is measured to be 0.806. If the path of the curvette is 1.07 cm, and the molar extinction coefficient is 5.362 M^-1.cm^-1, what is the concentration of the solution in MAnalyze the effect of concentration and path length on the absorption ofradiation in UV visible spectrophotometer.the absorbance of an equilibrium mixture of Fescn2+ was measured at 447nm and found to be 0.319. What is the equilibrium concentration of FeSCN2+?
- Compute for the linear equation of the following concentration and absorbance given in the table. SAMPLE CONCENTRATION ABSORBANCE 1 0.178 0.2127 2 0.274 0.2715 3 0.473 0.3892 4 0.574 0.4568 5 0.673 0.5327 6 0.864 0.6283 A. y=0.6169x+0.1031 B. y=0.5169x+0.1531 C. y=0.7169x+0.2031 D. y=0.6469x+0.1231Do read carefully and answer accordingly Chlorophyll a (a plant pigment) has the following molar extinction coefficient: εM662 = 8.4 x104 M-1 cm-1 A solution of different plant pigments has an absorbance of 0.632 at 662nm. Calculate the concentration of chlorophyll a in your sample assuming a light path length of 1 cm. -1.3 x 105 M -9.5 x 10-4 M -7.5 x 10-6 M -1.3 x 106 MThe observed fluorescence lifetime in the absenceof a quencher is 1.4 ns. In the presence of a quencher thefluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the fluorescence quantum yieldsin the presence and absence of the quencher. ¢,/¢,_0
- When benzophenone is illuminated with ultraviolet radiation it is excited into a singlet state. Th is singlet changes rapidly into a triplet, which phosphoresces. Triethylamine acts as a quencher for the triplet. In an experi ment in methanol as solvent, the phosphorescence intensity varied with amine concentration as shown below. A timeresolved laser spectroscopy experiment had also shown that the half-life of the fluorescence in the absence of quencher is 29μs. What is the value of the quenching rate constant kO ?Calculate the percent transmittance (%T) for a solution containing a colored species with an absorbance of 0.05305.What information is needed to determine the concentration of an unknown sample using spectroscopy? Group of answer choices A. Molar extinction coefficient derived from a standard curve, optimal wavelength determined from a wavelength scan and path length based on the length of the cuvette. B. Molar extinction coefficient derived from a wavelength scan, optimal wavelength determined from a wavelength scan and path length based on the length of the cuvette. C. Molar extinction coefficient derived from a standard curve, optimal wavelength determined from a standard curve and path length based on distance from light source to detector. D. Molar extinction coefficient derived from a standard curve, optimal wavelength determined from a wavelength scan and path length based on distance from light source to detector. E. Molar extinction coefficient derived from a standard curve, optimal wavelength determined from a standard curve and path length based on the length of the cuvette.…