A student wants to prepare a standard solution of BSA. They create a stock solution of BSA by add O.174 g of solid BSA powder into a 10 ml volumetric flask, then fills the flask to the line with 0.1M PBS buffer. The student uses a volumetric pipet to transfer 4 ml of stock solution into a new 10 ml volumetric flask, then fills the flask to the calibration line with 0.1M PBS buffer, and calls this Dilution 1. The student uses a micropipette to transfer 23 ul of Dilution 1 and 55 ul of 0.1M PBS butfer into a cuvette. Assuming the volumes are additive, what is the concentration of BSA in the cuvette in mg/mL? Report your answer with two places after the decimal.

A student wants to prepare a standard solution of BSA. They create a stock solution of BSA by add O.174 g of solid BSA powder into a 10 ml volumetric flask, then fills the flask to the line with 0.1M PBS buffer. The student uses a volumetric pipet to transfer 4 ml of stock solution into a new 10 ml volumetric flask, then fills the flask to the calibration line with 0.1M PBS buffer, and calls this Dilution 1. The student uses a micropipette to transfer 23 ul of Dilution 1 and 55 ul of 0.1M PBS butfer into a cuvette. Assuming the volumes are additive, what is the concentration of BSA in the cuvette in mg/mL? Report your answer with two places after the decimal.

Chemistry & Chemical Reactivity

10th Edition

ISBN:9781337399074

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions

Section4.9: Spectrophotometry

Problem 2.1ACP: Excess KI is added to a 100.0-mL sample of a soft drink that had been contaminated with bleach,...

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