a) The following analysis of P.pastoris was carried out after batch cultivation on 20 g/l glycerol (S)(C3H3O3) as the limiting substrate. with a starting cell concentration (X) = 0.5 gX/I Mass% Carbon = 50 Mass % Hydrogen = 7 Mass % Oxygen = 29 Mass % Nitrogen = 8 Mass% of cell that is "ash" = 6% Yx/s = 0.32 gX/gS X=biomass, S=glycerol a) Estimate the cell formula in terms of carbon (AW=12), hydrogen (AW=1), oxygen(AW=16), nitrogen(AW=14); AW = atomic weight in g/mol b) Determine the cell "molecular weight" and the degree of reductance of P.pastoris (degrees of reductance are C=4,H=1,0=-2,N=-3) c) Estimate the cell concentration at the end of the batch culture in gX/I

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The following analysis of P.pastoris was carried out after batch cultivation on 20 g/l glycerol (S) (C3H3O3) as the limiting substrate.
with a starting cell concentration (X) = 0.5 gX/I
a)
%3D
Mass% Carbon
50
Mass % Hydrogen = 7
%3D
Mass % Oxygen = 29
Mass % Nitrogen = 8
Mass% of cell that is "ash" = 6%
%3|
%3D
Yx/s = 0.32 gX/gS
X=biomass, S=glycerol
a) Estimate the cell formula in terms of carbon (AW=12), hydrogen (AW=1), oxygen(AW=16), nitrogen(AW=14); AW = atomic weight in g/mol
b) Determine the cell "molecular weight" and the degree of reductance of P.pastoris (degrees of reductance are C=4,H=1,0=-2,N=-3)
c) Estimate the cell concentration at the end of the batch culture in gX/I
Transcribed Image Text:The following analysis of P.pastoris was carried out after batch cultivation on 20 g/l glycerol (S) (C3H3O3) as the limiting substrate. with a starting cell concentration (X) = 0.5 gX/I a) %3D Mass% Carbon 50 Mass % Hydrogen = 7 %3D Mass % Oxygen = 29 Mass % Nitrogen = 8 Mass% of cell that is "ash" = 6% %3| %3D Yx/s = 0.32 gX/gS X=biomass, S=glycerol a) Estimate the cell formula in terms of carbon (AW=12), hydrogen (AW=1), oxygen(AW=16), nitrogen(AW=14); AW = atomic weight in g/mol b) Determine the cell "molecular weight" and the degree of reductance of P.pastoris (degrees of reductance are C=4,H=1,0=-2,N=-3) c) Estimate the cell concentration at the end of the batch culture in gX/I
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