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- THIS IS A 2 PART Question a. Is this a good streak? If so, explain why. If not, explain what you would change. b. Is this streak of a pure culture, a mixed culture, or a contaminated culture? Explain your response.You have been directed to take a sample from a growth-free portionof the zone of inhibition in the Kirby-Bauer test and inoculate itonto a plate of nonselective medium.a. What does it mean if growth occurs on the new plate?b. What if there is no growth?a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?
- What is the purpose of the cards used during exposure? What is the advantage of Bacillus species compared to this Serratia in this experiment?What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?explain why you would expect to see similarities in the types of colonies on the fomite plates and the handwashing plates?
- What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?You saw no color change in both the first step and second step of the Nitrate Reduction test. What is the result of this experiment? Also, explain what happens when an excessive amount of zinc is added in the second step of this test.- What is the purpose of the quadrant streak plate method?- How could this method be used in a clinical microbiology laboratory?- What happens if you do not flame the loop in between quadrants?
- Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could’ve caused this? Explain.How is the correct way of incubating culture plate media? Explain why is that so.Name and explain the culture media that can be used for cultivating non-specific and specific microorganism? b. can culture medium be placed in a freezer? why or why not