+ additional Pi +Pi no Pi added Time (min) CO, generated (mmoles)

Human Physiology: From Cells to Systems (MindTap Course List)
9th Edition
ISBN:9781285866932
Author:Lauralee Sherwood
Publisher:Lauralee Sherwood
Chapter17: Energy Balance And Temperature Regulation
Section: Chapter Questions
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Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data: 

a. Why is more CO2 generated upon the addition of phosphate?

b. You become worried that the flasks would burst open to the build up in gas pressure. What other molecules could you add to the extract to slow down CO2 generation even in the presence of glucose and Pi? 

+ additional
Pi
+Pi
no Pi added
Time (min)
CO, generated (mmoles)
Transcribed Image Text:+ additional Pi +Pi no Pi added Time (min) CO, generated (mmoles)
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