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- How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?
- Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.Order: Furosemide (Lasix) 30 mg, PO, bidAvailable: Furosemide 20 mg/2 mL How many milliliters of furosemide will you give per dose? __ mL Round to the nearest tenth.Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the following dilution scheme.
- how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow rate 4.75mL/min1200 ml of iv fluid must be administered to the patient over an 8 hour period. If the calibration rate is set a 8 drops/ ml, how many drops per minute will be administered to the patient?If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?
- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:The patient is ordered 150,000 units of Pfizerpen. Using 10mL of diluent, how many mL(s) will you administer? If rounding is requiredm, round to the nearest tenth. (Image for question is attached for more information)