how to calculate the experiment fow rate for this gel chromatographyExperiment flow rate?Flow rate4.75mL/min Time (sec)Volume of elution (mL) ( 1%bromophenol blue)Corrected OD10.23720.4083100.3424100.234100.3696130.4267170.2318180.3399140.31810140.23111120.34212150.22813170.33314180.40515150.231Total201

Given Values: Total volume eluted out of the gel filtration column = 15 ml The time taken in seconds…

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how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow rate 4.75mL/min

Principles Of Pharmacology Med Assist

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ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter1: Numerals And Fractions

Section: Chapter Questions

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Question

how to calculate the experiment fow rate for this gel chromatography

Experiment flow rate	?
Flow rate	4.75mL/min

Time (sec)
Volume of elution (mL) ( 1%
bromophenol blue)
Corrected OD
1
0.237
2
0.408
3
10
0.342
4
10
0.234
10
0.369
6
13
0.426
7
17
0.231
8
18
0.339
9
14
0.318
10
14
0.231
11
12
0.342
12
15
0.228
13
17
0.333
14
18
0.405
15
15
0.231
Total
201

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