Answer all questions and make it as short as possible. - After you are finished, list each step for the Gram staining procedure.- Which of the four steps is the most important and why?- What would happen to the cells if you use too much alcohol?- What would happen if you would not use enough alcohol?- What would happen if you left out the application of safranin?For each of the following stains, list each of the chemicals used (in order), briefly describe what you wouldobserve under the microscope, and list examples for each.Gram stain (list color and example for Gram + and Gram-)- Acid Fast (list color and example for Acid Fast + and -)- Negative Stain (list color of organism stained with negative stain)- Capsule Stain (Ilist color and example of organism with capsule)Spore stain (list color and example of spore and cell)

NOTE: AS PER OUR GUIDELINES WE NEED TO ONLY ATTEMPT FIRST QUESTION AND ITS PARTS , PLEASE RESUBMIT…

- After you are finished, list each step for the Gram staining procedure. - Which of the four steps is the most important and why? - What would happen to the cells if you use too much alcohol? What would happen if you would not use enough alcohol? - What would happen if you left out the application of safranin?

- After you are finished, list each step for the Gram staining procedure. - Which of the four steps is the most important and why? - What would happen to the cells if you use too much alcohol? What would happen if you would not use enough alcohol? - What would happen if you left out the application of safranin?

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter11: Filtration

Section: Chapter Questions

Problem 5RQ

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Similar questions

-What are the steps of gram staining -Which of the four steps is the most important and why?- What would happen to the cells if you use too much alcohol?- What would happen if you would not use enough alcohol?- What would happen if you left out the application of safranin?
You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.
In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?
What is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observed
After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explainwhy, the result you would want to observe and the interpretation of the result.
If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstained
For each of the following stains, list each of the chemicals used (in order), briefly describe what you would observe under the microscope, and list examples for each. - Gram stain (list color and example for Gram + and Gram -) - Acid Fast (list color and example for Acid Fast + and -) - Negative Stain (list color of organism stained with negative stain) - Capsule Stain (list color and example of organism with capsule) - Spore stain (list color and example of spore and cell)
What color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process? What are the primary and counterstain? What is the purpose for using a counterstain? What are some reasons a Gram positive cell might appear Gram negative? What color would a Gram negative cell be if you forgot to wash with ethanol and why? What color would a Gram negative cell be if you forgot to stain it with safranin and why?
Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each component work? (ie. What does the mordant do?)
Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)
Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple Staining
Briefly, in your own words, describe the steps of the gram staining procedure.