If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect Gram-positive bacteria to stain and what color would you expect Gram-negative bacteria to stain? Select one: O a. Gram-positive = red; Gram- negative = red b. Gram-positive = purple; Gram-negative = red || c. Gram-positive = red; Gram- negative = purple %3D O d. Gram-positive = purple; Gram-negative = purple %3D

If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect Gram-positive bacteria to stain and what color would you expect Gram-negative bacteria to stain? Select one: O a. Gram-positive = red; Gram- negative = red b. Gram-positive = purple; Gram-negative = red || c. Gram-positive = red; Gram- negative = purple %3D O d. Gram-positive = purple; Gram-negative = purple %3D

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?
If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstained
Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?
Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that step affect the end result?
Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few seconds
Answer this question xi) What is the most important step in the Gram staining procedure? Why?
Why is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
In the Gram staining procedure, how do the dyes able to distinguish Gram positive from Gram negative bacterial cells? What can you observe when you view a Gram stained-mixture of bacterial specimen?
Which stain is most frequently used to distinguish differences between thecell walls of medically important bacteria?a. simple stain. c. Gram stainb. acridine orange stain d. negativestain
Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol. Which of the following outcomes would you expect? Both Gram-positive and Gram-negative bacteria would appear reddish-pink Both Gram-positive and Gram-negative bacteria would appear purple Gram-positive bacteria would appear purple and Gram-negative bacteria reddish-pink Gram-positive bacteria would appear reddish-pink and Gram-negative bacteria purple Gram-positive bacteria would appear colorless and Gram-negative bacteria reddish-pink
The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see? a. none of these answers b. gram positive, endospore negative, acid-fast negative, capsule positive c. gram negative, endospore positive, acid-fast positive, capsule negative d. gram positive, endospore positive, acid-fast negative, capsule positive e. gram negative, endospore positive, acid-fast negative, capsule negative thank you!!
Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before staining