An enzyme has a Km of 8 M in the absence of an inhibitor and an apparent Km of 12 M in the presence of 3 M of an inhibitor. The Vmax is unaffected by the inhibitor. A. What type of inhibitor is this? B. Calculate KI for this inhibitor.

An enzyme has a Km of 8 M in the absence of an inhibitor and an apparent Km of 12 M in the presence of 3 M of an inhibitor. The Vmax is unaffected by the inhibitor. A. What type of inhibitor is this? B. Calculate KI for this inhibitor.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter32: Radiochemical Methods

Section: Chapter Questions

Problem 32.18QAP: The streptomycin in 500 g of a broth was determined by addition of 1.25 mg of the pure antibiotic...

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Question

An enzyme has a K_m of 8 M in the absence of an inhibitor and an apparent K_m of 12 M in the presence of 3 M of an inhibitor. The V_max is unaffected by the inhibitor.

A. What type of inhibitor is this?

B. Calculate K_I for this inhibitor.

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