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Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter8: Thin-layer Chromatography: Analyzing Analgesics And Isolating Lycopene From Tomato Paste

Section: Chapter Questions

Problem 10Q: A TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the...

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Concept explainers

Micelles and Biological Membrane

Micelles are the type of lipid molecules that organize themselves in spherical shape in aqueous solutions. The production of the micelle is a reply to the amphipathic behavior of fatty acids. It means that they consist of both hydrophobic regions and hydrophilic regions. In micelles, polar head groups (hydrophilic groups) produce an outer layer associated with water whereas nonpolar tails (hydrophobic groups) are organized in the interior.

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You need to separate a mixture of dyes on an agarose gel. You have been provided with 5X TAE (Tris acetate buffer) The electrophoresis tank needs 250 ml of buffer and the gel volume required is 50 ml. Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment. Explain how you will prepare the buffer and gel 4m

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