In a Bradford assay, 11 µL of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 3 6 conc of BSA (rg/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

In a Bradford assay, 11 µL of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 3 6 conc of BSA (rg/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter15: Molecular Luminescence Spectrometry

Section: Chapter Questions

Problem 15.7QAP

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You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.187. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
A standard curve for glucose analysis was prepared.The slope (m) was found to be 1.341 while the y-intercept (b) was -0.333. Calculate the concentration (x) of glucose having an absorbance (y) of 0.151. 0.067 0.361 0.858 0.136
1 mL was taken from the sample filtrate and mixed with 13 mL pure water and 4 mL sulfomolybdic acid and 2 mL dilute SnCI2 solution by adding it, and after waiting for 15 minutes, the absorbance of the resulting solutions against pure water was read at 520 nm. if the function of the calibration graph obtained with standard phosphorus solutions of 0.5-2.5 mg/mL is y= 0.245x + 0.107 and the absorbance of the serum sample is 0.342, how many grams of phosphorus is the amount in the sample?
As part of an analytical chemistry laboratory course, a student measured the Ca2+ content in two water samples, city-supplied drinking water and well-supplied drinking water, using two different analytical methods, flame atomic absorption spectrometry (FAAS) and EDTA complexometric titration. The results of this experiment are given in the table as the mean Ca2+concentration (?¯) and standard deviation (?) in parts per million (ppm). Each sample was measured five times (n=5) by each method. Method City-Supplied Drinking Water (?¯±?x¯±s) Well-Supplied Drinking Water (?¯±?x¯±s) FAAS 57.57±0.68 ppm 64.77±0.70 ppm EDTA titration 58.32±0.96 ppm 65.62±0.97 ppm Method Comparison: For each drinking water sample (city and well), compare the Ca2+ content measured by FAAS and EDTA titration. Calculate the ? value for each sample. Do the methods produce statistically different results at the 95% confidence level when measuring the Ca2+content of the city-supplied drinking water? Do the…
A 20 ppm solution of a DNA molecule (unknown molecular weight) isolated from Escherichia coli was found to an absorbance of 0.80 in a 2-cm cell. Calculate the absorptivity of the molecule.
The standard curve was made by spectrophotographic analysis of equilibrated iron(III) thiocyanate solutions of known concentration. You are asked to analyze a Fe(SCN)2+Fe(SCN)2+ solution with an unknown concentration and an absorbance value of 0.4150.415 . The slope-intercept form of the equation of the line is ?=4558.4?+0.0147y=4558.4⁢x+0.0147 . The unknown was analyzed on the same instrument as the standard curve solutions at the same temperature. What is the Fe3+Fe3+ concentration of the unknown solution?
The predominate form of caffeine at pH 9.4 is neutrally charged. If nitromethane, a compound often used as neutral marker, was added to the sample and standard CE vials, predict the impact on the electropherogram, the peak areas, and the determination of caffeine, aspartame and benzoic acid concentrations?
The concentration of phenol in an aqueous solution is 10 ug/mL. The absorbance is found to be 0.209 when this solution is placed in a 1.00 cm cuvette and 258 nm radiation is passed through Calculate the specific absorptivity, including units, of phenol? What will be the absorbance if the solution is diluted to 5 ug/mL? What will be the absorbance if the path length of the original solution is increased to 5.00 cm?