3. Internal Standard. A solution containing 3.47 mM X (analyte) and 1.72 mM S (standard) gave peak areas of 3.473 and 10.222, respectively, in a chromatographic analysis. Then 1.00 mL of 8.47 mMS was added to 5.00 mL of unknown X, and the mixture was diluted to 10.00mL. This solution gave peak areas of 5.428 and 4.431 for X and S, respectively. (a) Calculate the response factor of the analyte. (b) Find the concentration of S (mM) in the 10.0 mL of mixed solution. (c) Find the concentration of X (mM) in the 10.0 mL of mixed solution. (d) Find the concentration of X in the original solution.

Q: One microliter of 0.20 μg/mL0.20 μg/mL benzene is injected into a gas chromatograph using splitless…

A:

Q: What Are the Main Differences Between High Performance Liquid Chromatography and Gas Chromatography?

A: The high performance liquid chromatography and gas chromatography are used to determine the…

Q: Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave…

A:

Q: [2] How might a better chromatographic separation of solutes be accomplished? [3] What would happen…

A: Chromatography is one of the most important techniques that is used analytical techniques. It is…

Q: 1. What's the slope of the calibration curve? 3.95 x 10-5 4.17x10-5 1 20484 25257 2. What's the…

A: The linear regression equation of the calibration curve is: Y = 25257X - 114.32

Q: A solution containing 2.30 mM of X (analyte) and 1.15 mM of S (standard) gave peak areas of 1728 and…

A: Given,   known Amount of X = 2.30 mM, amount of S = 1.15 mM  Peak area of X is 1728 and that of S is…

Q: A spectrophotometric method for the quantitative determination of the concentration of Pb2+ in blood…

A:

Q: A 10.00-g sample containing an analyte was transferred to a 250-mL volumetric flask and į diluted to…

A: Solution - According to the question - Given - In the spiked sample, the signal is due to 10 ml of…

Q: Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1…

A: The given process is the oxidation of benzoin to benzil and the purification of the product through…

Q: (3) In a titration, the % analyte is computed from the following equation: Vol (titrant) x M…

A:

Q: A banned pesticide, DDT, is analyzed through gas chromatography. A DDT standard is diluted…

A: In analytical chemistry a calibration curve or standard curve, is plotted to determine the…

Q: A solution with 0.083 7 M X and 0.050 0 M S9 gave areas AX = 423 and AS9 = 372. Then 10.0 mL of 0.05…

A: Given: A solution having 0.0837 M X and 0.0500 M S’ showed peak areas AX = 423 and AS’ = 372,…

Q: Samples X and Y were analysed for Na by flame emission photometry. A series of standard solutions…

A: A question based on analytical process that is to be accomplished.

Q: To quantify the amount of ethanol in a beverage, a technician prepared the first solution which…

A: Gas chromatography (GC) is an analytical technique that is used for detection and quantification of…

Q: A second spectrophotometric method for the quantitative analysis of Pb2+ in blood yields an Ssamp of…

A: The value of sensitivity of the method for the analyte (kA) is calculated using sample signal…

Q: A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas…

A: We are given for peak A of methyl benzoate of 1.37 mg/ml gave an area of 301, and peak B of butyl…

Q: A volume of 250 ml of a 0.05 M solution of a reagent of formula weight (relative molecular mass) 40…

A:

Q: A student researcher performed a chromatographic separation of caffeine and aspartame. The retention…

A:

Q: From a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL.  From this, a 5-mL aliquot was…

A: The diluted conc. of any solution can be calculated by using the dilution formula:…

Q: A sample of anhydrous Na2CO3 (FM = 105.989) is suspected to be contaminated with either NaHCO3 (FM =…

A: Given information: Mass of sample = 0.7483 g Volume of solution = 50.00 mL Normality of HCl = 0.0125…

Q: A student researcher performed a chromatographic separation of caffeine and aspartame. The retention…

A: Given tc = 209.6 sωc = 14.4 sta = 260.0 sωa = 21.2 sd = 22.1 s

Q: A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas…

A: The given type of aanylysis called the Single Point Internal Standard method. Internal response…

Q: Internal standard. A solution containing 3.47 mM X (analyte) and 1.72 mM S (standard) gave peak…

A: (a) Calculate the response factor for X: Response factor=Area of analyte peak/Concentration of…

Q: Gas chromatography cannot analyze mixtures due to the sensitivity of the instrument. O True O False

A: The statement, "Gas chromatography cannot analyze mixtures due to the sensitivity of the instrument"…

Q: The peak integrations of a chromatogram resulting from a GC analysis of a mixture containing…

A: Area under the curve of GC is correspond to mass of that component.

Q: A mixture of three components is separated using paper chromatography, and each component is…

A: Given: Absorbance of standard solution- Compound A = 0.72 Compound B = 0.55 Compound C = 0.80 For…

Q: A mixture of three components is separated using paper chromatography, and each component is…

A: Given: Absorbance of standard solution- Compound A = 0.72 Compound B = 0.55 Compound C = 0.80 For…

Q: 25 ml of pickled brine was taken from an apple pickled with vinegar and diluted to 500 ml, 25 ml of…

A: The dilution law is shown below: N1V1 = N2V2 where; N1 = normality of unknown acid/base V1 = volume…

Q: Standardization of EDTA Solution Weight of pure CaCO3: 0.2517 g % Purity of CaCO3: 98.0% Total…

A: The experiment data given is, Trial 1 2 3 The volume of standard CaCO3 solution (ml)  25.00…

Q: 25 nm, which çan be used for the spectrophotometric determination of ammonia. OCF 2 OH + NH3…

A: Solution -

Q: A stock solution of 0.225 +/- 0.003 M NaNO2 was transferred to a 100 mL volumetric flask (class A)…

A: Given that, Concentration of stock NaNO2 = 0.225 ± 0.003M 7 ml of stock NaNO2 is added to 100 mL…

Q: In a chromatographic analysis, a solution containing 0.0837M toluene and 0.0666M xylene resulted in…

A: Response factor can be represented as

Q: Q3: In a chromatographic analysis, a solution was prepared with 0.0837 M Octane (A) and 0.0666 M…

A: In the given question we have to calculate the concentration of octane in unknown sample.

Q: 2. A spectrophotometric method for the quantitative determination of the concentration of Pb in…

A: Given:-             To find the concentration of the lead in the blood sample and its equation is:…

Q: Internal standard (As , Cs ), analyte with known concentration (Ax , Cx ) In a chromatographic…

A: Formula used: Area of analyte signalConc of analyte= F×Area of standard signalConc of…

Q: A standard sample contains 15.0 mM of analyte and 25.0 mM of internal standard. The analysis of the…

A: Internal standard method: In analytical chemistry addition of chemical substance to a sample is in…

Q: An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The…

A: To find out the mobile phase velocity, number of theoretical plates and height equivalent to…

Q: Doris was studying the concentration of Guaifenesin (MM 198.216 g/mol) in Exciting Brand Mucus and…

A:

Q: In a chromatography experiment, a solution containing 0.083 7 M X and 0.066 6 M S gave peak areas of…

A: The equation to calculate the concentration of unknown using internal standard from a chromatogram…

Q: With the following information for a GC analysis using an internal standard, calculate the…

A:

Q: A method of quantitative determination of the concentration of constituents of a sample analyzed by…

A: Gas chromatography It is a chromatographic technique used in laboratories in which mixture of…

Q: A solution containing 3.51 mM X (analyte) and 1.67 mM S (internal standard) gave peak areas of 3505…

A: Given :       Solution containing 3.51 mM X(analyte) & 1.67 mM S(internal standard)       gave…

Q: A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase…

A: (a)  The mathematical expression for calculating the number of plates is: N=16(tRW)2

Q: Explain temperature programming for gas chromatography and what its benefits.

A: Temperature programming is used to run a large fraction of all gas chromatography. In this the…

Q: A simple paper chromatography experiment involved the analysis of the food dyes in lollipop samples.…

A: This can be explained by Retention Factor value.

Q: Two components of a 12-mg sample are adequately separated by chromatography through a column 1.5 cm…

A: Given: 1) There are two components of a 12-mg sample = Small column mass 2) The column is 1.5 cm in…

Q: der the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here.  The elution…

A: Given data: Elution time for unretained solute = 1.06 min Length of column = 30 m Diameter of column…

Q: Often the analyst will compare known quantities of analyte to unknown quantities of the material to…

A: Analytical chemistry is a branch of chemistry that deals with the separation and identification of…

Q: . A third spectrophotometric method for the quantitative analysis of Pb in blood yields an Ssamp Of…

A: Concentration of Pb2+ in original sample can be calculated using standard addition method. In which…

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.