Bacteria count Shown is a typical graph of the number N of bacteria grown in a batch culture as a function of time 1. Describe what you think is happening during each of the four phases. NA
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- If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli? (optimum temperature 20o-40oC; most human pathogens)If you altered the conditions under which bacterial growth normally occurs (i.e. increase the temperature of incubating a culture from 35 to 40°C), what effect would this have on the bacterial growth curve of the organism under study?A new intestinal isolate of E. coli is known to have a generation time of 12 minutes. If a culture contained 3, 221,225,472 bacterial cells after 6 hrs how many bacteria must have been inoculated into the culture at time 0.
- You have just measured the OD of your E. coli (which is in log-phase growth) and it is at 0.15. You are doing a procedure that requires you to centrifuge E.coli once it reaches an OD of 1.20 do you have time to run to the bathroom? In other words, (i) explain the logic, and (ii) show the math to illustrate how you know what time you need to return to lab to centrifuge those bacterial cells.How many colonies would you expect to count at each 5-fold dilution (5-1 until 5-6), when you have 338,000 bacteria present per gram of a meat sample. Given you inoculate 1 mL of each of these dilutions.A sample of raw ground beef initially contained 25 E. Coli bacteria. After six hours, there are 102 400 cells in the sample. What is the growth rate of the E. coli population? Show all work. Express your answer using scientific notation in the form of xyz 10 d #bacteria/hr.
- Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C Total time of exposure (minutes): Number of Microbial Cells Present: 0 100 1 80 3 50 4 42 6.5 26 13 10 21 0If a culture of E. coli you are tracking grows from 5 x 103 cells/ml to 9 x 107 cells per ml in 4 hours, what is the approximate generation time of your E. coli? how can i slove this step by step a. About 14 minutesb. About 17 minutesc. About 20 minutesd. About 23 minutese. About 30 minutes
- A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the generation time is 1 hour. How many E. coli are on the slant in 6 hours? If the slant was initially put in the 37C incubator, with a 20 minute generation time, how many more cells would have grown in the same time period?Calculate the doubling time of a culture of E. coli that is started with 3x105 CFU/mL and grows to 4.6x108 CFU/mL in 3 hours. Be sure to use units in your answer.In the Messelsohn-Stahl experiment, there should be ______ band(s) in the centrifugation tube after two generations of bacterial growth in normal nitrogen. Group of answer choices None of these One light and one medium density Two light and heavy Three light bands One heavy