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PROBLEMS 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol I'): Initial velocity (umol I' min"): 5.0 6.67 10.0 233 20.0 323 40.0 400 147 182 Calculate Km and Vmax 7.2 Malate dehydrogenase catalyses the reaction: (S)-malate + NAD = oxaloacetate + NADH + H' The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD'] (mmol r") t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.033 0.056 0.079 0.102 0.116 0.138 0.154 0.168 0.175 0.122 2.0 0.036 0.063 0.089 0.099 0.138 2.5 3.33 0.040 0.043 0.069 0.128 0.150 0.075 0.108 0.140 0.163 5.0 0.047 0.084 0.121 0.137 0.158 0.177 0.184 10.0 0.052 0.095 0.180 0.192 0.200 Calculate Km and Vmax ·

PROBLEMS 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol I'): Initial velocity (umol I' min"): 5.0 6.67 10.0 233 20.0 323 40.0 400 147 182 Calculate Km and Vmax 7.2 Malate dehydrogenase catalyses the reaction: (S)-malate + NAD = oxaloacetate + NADH + H' The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD'] (mmol r") t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.033 0.056 0.079 0.102 0.116 0.138 0.154 0.168 0.175 0.122 2.0 0.036 0.063 0.089 0.099 0.138 2.5 3.33 0.040 0.043 0.069 0.128 0.150 0.075 0.108 0.140 0.163 5.0 0.047 0.084 0.121 0.137 0.158 0.177 0.184 10.0 0.052 0.095 0.180 0.192 0.200 Calculate Km and Vmax ·

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter13: Enzymes—kinetics And Specificity
Section: Chapter Questions
Problem 16P
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PROBLEMS 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol I'): Initial velocity (umol I' min"): 5.0 6.67 10.0 233 20.0 323 40.0 400 147 182 Calculate Km and Vmax 7.2 Malate dehydrogenase catalyses the reaction: (S)-malate + NAD = oxaloacetate + NADH + H' The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD'] (mmol r") t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.033 0.056 0.079 0.102 0.116 0.138 0.154 0.168 0.175 0.122 2.0 0.036 0.063 0.089 0.099 0.138 2.5 3.33 0.040 0.043 0.069 0.128 0.150 0.075 0.108 0.140 0.163 5.0 0.047 0.084 0.121 0.137 0.158 0.177 0.184 10.0 0.052 0.095 0.180 0.192 0.200 Calculate Km and Vmax ·
Transcribed Image Text:PROBLEMS 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol I'): Initial velocity (umol I' min"): 5.0 6.67 10.0 233 20.0 323 40.0 400 147 182 Calculate Km and Vmax 7.2 Malate dehydrogenase catalyses the reaction: (S)-malate + NAD = oxaloacetate + NADH + H' The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD'] (mmol r") t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.033 0.056 0.079 0.102 0.116 0.138 0.154 0.168 0.175 0.122 2.0 0.036 0.063 0.089 0.099 0.138 2.5 3.33 0.040 0.043 0.069 0.128 0.150 0.075 0.108 0.140 0.163 5.0 0.047 0.084 0.121 0.137 0.158 0.177 0.184 10.0 0.052 0.095 0.180 0.192 0.200 Calculate Km and Vmax ·
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