ou have given two proteins with a pI of 4.5 and pI of 7.7? Using an anion-exchange column, how can these two proteins be separated. What buffer could you prepare?
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You have given two proteins with a pI of 4.5 and pI of 7.7? Using an anion-exchange column, how can these two proteins be separated. What buffer could you prepare?
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- You are given two proteins with a pI of 4.5 and pI of 7.7. In an example of an anion-exchange column, can you use a buffer to separate these two proteins? What buffer protein can be used to separate the two proteins mentioned above?What is the pH of a buffer of a buffer system that contains a weak acid of .19 M and conjugate base of .12 M ? The Ka of the acid is 5.6x 10 -6 pH= pKa + log ( [A- ]/[HA]) please show me step by stepIf you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
- Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.Tomato juice has a OH- concentration equal to 1 X 10 -10, what is the pH of this solution?For a weak acid with a pKa of 6.0, calculate the ratio of conjugate base to acid at a pH of 5.0
- what is the molar concentration of 80g of glucose dissolved in sufficient water to make two liters of solution.Three buffers are made by combining a 1M solution of acetic acid and a 1M solution of sodium acetate in the ratios shown in the table below. Which of these statements is true regarding the prepared buffers? (Ka= 1.7x10-5) pH of buffer 1 < pH of buffer 2 < pH of buffer 3 pH of buffer 1 = pH of buffer 2 = pH of buffer 3 pH of buffer 1 > pH of buffer 2 > pH of buffer 3 pH of buffer 1 = pH of buffer 2 > pH of buffer 3 pH of buffer 1 > pH of buffer 2 = pH of buffer 3Based on the information in Table 3.2, is there any amino acid that could serve as a buffer at pH 8? If so, which one?
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