Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography
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Briefly explain the principle behind the following techniques:
(a) ion-exchange chromatography
(b) gel filtration chromatography
(c) affinity chromatography
(d) Use three diagrams to illustrate the separation of three different proteins by these
methods.
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- Briefly explain how cation exchange chromatography can used in protein separation?What further testing should be done to extract and compare the composition of food dyes used In two types of candies.(except in paper chromatography)Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.
- what is Optical rotatory dispersion and circulatory dichroism method for protein binding?Which of the following statement is false? a. Component with the strong attraction to the support move more slowly than component with weak attraction. b. Chromatography technique used to separate only colored compounds. c. On TLC, single spot does not mean or guarantee a single substance (compound). d. Iodine vapor and UV- lamp are methods used for visualization uncolored compounds.As distillation method is used to seperate a homogenous mixture, which kind of mixtures chromatography can seperate? Give some example.
- In the paper chromatography experiment, the filter paper is the stationary phase and water is the mobile phase False, cellulose is the mobile phase and salt solution is the stationary phase False, adsorbed water is the stationary phase and salt solution is the mobile phase False, salt solution is the mobile phase False, adsorbed water is the mobile phase and salt solution is the stationary phaseIs thin-layer chromatography a type of normal-phase chromatography or a type of reverse-phase chromatography? Define these two classifications.Suggest two possible applications for paper chromatography other than the separation of dyes or the identification of metal ions.
- In chromatography, the compound that is least soluble in the mobile phase a) Travels the furthest away from the line of origin b) Stays closest to the line of originTell me about the Laboratory experiment: Purifying Protein by Column Chromatography Thank you^^1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.