+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute

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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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+Protein
Mr
pl
А
12,000
28,000
42,000
66,000 (a;)
103,000 (a2)
111,000
3
8.4
C
7.2
9
E
5
F
4.2
Predict how the proteins listed above will behave in a variety of separation and
characterization procedures.
For each chromatographic technique (you may assume that the protein remains
“native," or non-denatured), list the order in which the proteins will elute (come of
the end of the column):
Size Exclusion Chromatography, pH 6.8
First
Last
Anion Exchange Chromatography, pH 9, using a salt gradient to elute
First
Last
Transcribed Image Text:+Protein Mr pl А 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 3 8.4 C 7.2 9 E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute First Last
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