Calculate how many moles of ATP can be made in standard conditions when 1 mole of protons flow back into the mitochondrial matrix through the ATP synthase. Assume that: i) the matrix pH is 7.8, ii) the cytosolic pH is 7.2, iii)ΔE=0.168 V (inside negative), iv) T=37°C, and v) the standard free energy change for the hydrolysis of 1 mole of ATP is -31.3 kJ/mol.

Calculate how many moles of ATP can be made in standard conditions when 1 mole of protons flow back into the mitochondrial matrix through the ATP synthase. Assume that: i) the matrix pH is 7.8, ii) the cytosolic pH is 7.2, iii)ΔE=0.168 V (inside negative), iv) T=37°C, and v) the standard free energy change for the hydrolysis of 1 mole of ATP is -31.3 kJ/mol.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter27: Metabolic Integration And Organ Specialization

Section: Chapter Questions

Problem 4P

See similar textbooks

Similar questions

For the mitochondrial membrane described in pic, how many protons must be transported to provide enough free energy for the synthesis of 1 mol of ATP (assuming standard biochemical conditions)?
1 a) What is meant by the ATP currency exchange ratio? Why does the oxidation of mitochondrial FADH2 generate one less ATP than oxidation of mitochondrial NADH? b) If 12 H+ are moved across the inner mitochondrial membrane by NADH oxidation, and each ATP synthesized requires 3 H+ to move through ATP synthase, why are only 3 ATP molecules produced by oxidation of each NADH?
Under standard conditions, is the oxidation of ubiquinol (Coenzyme Q) by O2 sufficiently exergonic to drive the synthesis of ATP? If yes, how many ATP can be synthesized assuming 100% efficiency?
Assuming that the pH of the IMS is 1.4 units lower than the pH of the matrix, the free energy yield associated with the transport of a proton from the mitochondrial intermembrane space (IMS) to the mitochondrial matrix is △ G of the reaction is 8.310 KJmol-1 What is the minimum number of protons that must enter the mitochondrial membrane from the IMS in order to synthesize one molecule of ATP?
Under standard conditions, NADH reoxidation by the electron-transport chain has a free-energy change equal to –220 kJ/mol. With 100% efficiency, how many ATP could be synthesized under standard conditions? What is the "actual" efficiency given these numbers?
Calculate the number of usable ATP molecules produced per pair of electrons transferred from NADH to oxygen, if (i) five protons are pumped across the inner mitochondrial membrane for each electron passed through the three respiratory enzyme complexes, (ii) three protons must pass through the ATP synthase for each ATP molecule that it produces from ADP and inorganic phosphate inside the mitochondrion, and (iii) one proton is used to produce the voltage gradient needed to transport each ATP molecule out of the mitochondrion to the cytosol where it is used.
Considering that 22.4 kJ/mole is required to transport 1 H+ across the mitochondrial membrane,how much energy is available for proton translocation from the oxidation of FADH2 at Complex II? Answer: 134 kJ/mol Can you please explain why the answer would be 134 kJ/mol?
Calculate the standard free-energy change of the reaction catalyzed by theenzyme phosphoglucomutase, given that, starting with 20 mM glucose 1-phosphate and no glucose 6- phosphate, the final equilibrium mixture at 25 °C and pH 7.0 contains 1.0 mM glucose 1-phosphate and 19 mM glucose 6-phosphate. Does the reaction in the direction of glucose 6-phosphate formation proceed with a loss or a gain of free energy?
What Is the P/O Ratio for Mitochondrial OxidativePhosphorylation?
Show the path of electrons from ubiquinone (Q or coenzyme Q) to oxygen in the mitochondrial respiratory chain. One of the two compounds (Q and O2) has a standard reduction potential (E'°) of 0.82 V, and the other, 0.045 V. Which value belongs to each compound? How did you deduce this?
Draw and label a lipid bilayer containing the large mitochondrial trans-membrane protein complexes representing complex I, II, III, and IV, and ATP Synthase. Label your diagrams with the correct names for the membranes, Make two more of these drawings. Label the first one mitochondrial electron source and using a different ink color, indicate the source(s) of electrons. Label the second mitochondrial energy source, and indicate the source of energy driving the electron transport chain. In the third, label the mitochondrial electron acceptor in its proper position. Also include ATP synthase (in its correct orientation-the spherical part is on the opposite side of the membrane as the H+ reservoir). Use a different color of ink to show where the protons (H+) are pumped to drive ATP synthesis. also indicate where ATP is generated and were the power is stored to generate ATP. NOW, do the same thing with chloroplasts. Draw and label a lipid bilayer containing the large photosynthetic…
If a reaction has a ΔG°′ value of at least −30.5 kJ · mol−1, suffi -cient to drive the synthesis of ATP (ΔG°′ = 30.5 kJ · mol−1), can it still drive the synthesis of ATP in vivo when its ΔG is only −10 kJ · mol−1? Explain.