Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 ml 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:
Q: when testing unpasteurized milk you get a colony count of 118 colonies on plate A and 110 colonies…
A: As the name suggest, the serial dilution is a series of the sequential dilution performed to convert…
Q: You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate…
A: Serial dilution is method of diluting a bacterial culture in succession. The process reduces the…
Q: A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of…
A: Nelson Somogyi Method: Reducing sugars are sugars that have a reducing property rendered by a…
Q: Explain the mechanism leading to the given reactions in the inoculated biochemical media. 1.…
A: Triple Sugar Iron test (TSI) is a microbiological test carried out in differential medium to test a…
Q: Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you…
A: There are few important points : ELISA : Enzyme linked immuno sorbent assay is used in diagnosing…
Q: Tables Table 1: Time Required for Methylene Blue Color Change Milk Sample Start Time/Date (Step…
A: Methylene blue Is the blue color dye used to check the presence of bacteria in raw and pasteurized…
Q: What are the mechanisms of samples separation work in Thin layer Chromatography?
A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…
Q: assuming that serial dilution was carried out in a laboratory experiment in 6 tubes with 9 ml of…
A: Introduction: The stepwise dilution of a solution is referred to as serial dilution. Usually, the…
Q: A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate,…
A: Colony forming unit (CFU) is a unit that tells us the amount of viable cells present in the given…
Q: 1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo…
A: Introduction In Virology, A Plaque-forming Unit (PFU) Is A Metric That Describes How Many Virus…
Q: You already collected samples for the following tests, in what section of the laboratory will you…
A: Clinical laboratories are facilities of healthcare, where clinical tests are performed on patients…
Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…
A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…
Q: You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your…
A: The concentration of bacteria depends on many factors like inoculum concentration, incubation…
Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: Five ml of bacterial culture is added to 45 ml of sterile diluent. From this suspension, the…
A: INTRODUCTION Dilution factor It is a solute to solvent ratio.
Q: theraphy, What are the drawbacks of this technology? Enumerate at least 2and briefly explain ypur…
A: Gene therapy can be defined as the introduction of a new DNA into an old DNA to bring about genetic…
Q: Calculate the viral titer of an experiment performed yielding a pfu of 279 from plating 1.0 ml of…
A: Virus titre calculation is a complicated way that a scientist is counting the “number of viruses”…
Q: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in…
A: 1*Erlenmeyer flasks are used to contain liquids and for mixing and heating and cooling, incubation…
Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…
A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…
Q: Define D-dimer test and how to interpret the test. The reference range is in the table below Test…
A: Platelets are the cellular components of the blood that are involved in blood clotting, which is a…
Q: On a spread plate, you count 50 colonies. The plate was created from a 10^-2 dilution and 0.2 mL of…
A: Microbial culture refers to the method of letting the micro-organisms multiplying in the culture…
Q: 1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic…
A: Two methods widely used for bacterial identification in the laboratories are: Chemical assays PCR…
Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…
A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…
Q: Below I have attached the image of gram stain. -We need to find the name of the compound. And after…
A: Gram negative bacteria - The bacteria which are unable to retain crystal violet stain in the process…
Q: The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text…
A: Osmolarity is defined as the number of moles of solute present in 1 litre of solution. When a…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: 100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of…
A: An artificial nutrient medium contains nutritional components like sugars, salts, amino acids, etc.…
Q: Thank you, 3
A: Ans : CFU/ml = 1.25 * 106 Given : * Dilution factor = 1/1000 or 10-3 * Sample plated = 0.1ml *…
Q: Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the…
A: In the laboratory blood samples are used to detect various disease conditions. There are different…
Q: With the constants you have for each objective, compute for the sizes of the given cells below.…
A: As we know that cell is the structural and functional unit of life it performing all the essential…
Q: A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the…
A: CFU means colony forming unit. It is a measure of viable bacterial or fungal cells. In case of…
Q: You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent.…
A: The dilution factor can be defined as the ratio of Volume of the initial solution to the volume of…
Q: How do you know if you have a ‘countable’ plate when doing a dilution series? Two plates…
A: The concentration of stock solution can be calculated by first diluting the stock into a suitable…
Q: Plating 0.1 mL of a sample diluted by a factor of 10^(-3) produced 43 colonies. What was the…
A: The question asks the original concentration (OCD) is to be identified in an undiluted…
Q: CFU/mL
A: in calculation via conventional formula , CFUs/ml in 2nd dilution are greater than 1st dilution…
Q: If, when using 10X ocular and a 20X objective, the field size is 2 mm. The approximate field size…
A: Ocular magnification - This is the magnification that can be viewed by the ocular lenses, the lenses…
Q: 1) Write your dilutions as a rational number AND in scientific format? What was your total dilution…
A: Serial dilution is a process in which a sample is taken and diluted through a series of standard…
Q: Discuss the functions of the following components in performing gel electrophoresis;…
A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…
Q: Refer to the following diagram: 100 ul 100 μL 100μι 10 μι 10 μι 100 μι 100 μL Volume to transfer 900…
A: Colony forming unit is used to determine the concentration of the microorganism in the test sample.…
Q: What are the different manufacturing methods of Suppositories dosage form? Explain heat molding…
A: Suppositories are the solid medication that delivers drug in rectum ,vagina and or urethera. Most…
Q: What is the purpose of Biuret’s test? In biuret’s test, does the color in each test tube containing…
A: There are different biomolecules, including carbohydrates, protein, lipids, etc., are present. The…
Q: Which of the following methods for determining protein concentration is most commonly used? O Lowry…
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: 1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to…
A: A bacteria culture is described as a type of test that is used to confirm whether you have a…
Q: 5ml of 1 x 10° cells/ ml cell suspension is required to be prepared for seeding on the cell culture…
A: The hemocytometer is a counting chamber or slide used for the purpose of cell counting. It was…
Q: In RPR test, Why is it necessary to rotate the slide/card?
A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.
Q: Assuming that serial dilution was carried out in a laboratory experiment in 6 tubes with 9 ml of…
A: Serial dilution method : It is a series of sequential dilutions used to reduce a dense culture of…
Q: Calculate the concentration of bacteriophage in the origina! culture from the following data. Be…
A: Bacteriophage -- Also called as bacterial virus or phage. Bacteriophages are obligate intracellular…
Q: Give the ideal observation for the positive results of the following samples to complete the table…
A: Proteins or peptides are composed of twenty standard amino acids attached via peptide bonds. These…
Trending now
This is a popular solution!
Learn your way
Includes step-by-step video
Step by step
Solved in 2 steps with 2 images
- In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
- A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.Below is a set of data for a serial dilution/plate count experiment. Based on this data what is the calculated concentration of bacteria in CFU/mL in the original culture? The OD600 of a bacterial culture that's been diluted in half is 0.842. What is the estimated population of cells in the original undiluted culture? asapThe following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.
- You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000"If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000
- A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 61 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 278 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture? Express your answer to two decimal places using scientific notation. In scientific notation 540 would be written as 5.40*10^2. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transferred, a dilution is being performed. Any time more than 1 mL is transferred, a concentration is being performed. Include the trailing zero so there are two decimal places Canvas expects a single digit before the decimal point. 5.40*10^2 is how Canvas expects 540 to be formatted in scientific notation 54.00*10^1 would be marked wrong. 0.54*10^3 would be marked wrong. A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the…volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)