Which of the following methods for determining protein concentration is most commonly used? O Lowry assay O Smith copper/bicinchonic assay Bradford Dye method
Q: explain how the following genotypic and phenotypic methods of microbe identification is done. Use…
A: Genotypic identification of microbes is an alternative to the established phenotypic methods of…
Q: Provide the function of the following in blotting techniques: Nitrocellulose paper paper weight…
A: Function of Nitrocellulose paper in blotting: • Nitrocellulose paper is used in protein blotting…
Q: If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum…
A: Lawry essay is used to determine the protein level in a solution as it shows a color change…
Q: In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was…
A: Coating buffer in ELISA is a mix of sodium carbonate and bicarbonate at ph 9.4. This gives charge to…
Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…
A: Biochemical pathway will be as- MM- MM+C - MM+A - MM+B - CM Hint- the more the positive signs of…
Q: FNA
A: The above test is called the COAGULASE test. The coagulase test is a experiment to determine whether…
Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…
A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…
Q: Which of these is not considered a method of DNA extraction? organic filtration differential…
A: DNA (deoxyribonucleic acid) extraction is a procedure to isolate DNA from the cells. It is a method…
Q: Since the development of the calcium chloride transformation procedure, other procedures have been…
A: Bacterial transformation is the transfer of DNA released from a donor bacterium into the…
Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…
A: Auxotrophic microorganisms cannot produce a certain organic chemical essential for their growth. The…
Q: Which of the following is similar to a block of highly purified “jello-like” substance that is not…
A: Micropippete is laboratory equipment used to transfer small quantities of liquid. Restriction…
Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: DNA concentration is 3.75 ng/ ul = the dilution factor is 1:______ we add____ uL dna extract to ____…
A: DNA shows an absorbance maxima at 260 nm and the absorbance is proportional to the concentration of…
Q: Which reagent removes contaminant proteins from DNA solution? Sodium dodecyl sulfate (SDS) O Sodium…
A: A major goal of the nucleic acid isolation is the removal of proteins . The seperation of nucleic…
Q: The elimination of several steps in the ELISA could be accomplished if the primary antibody was made…
A: The enzyme-linked immunosorbent assay (ELISA) is a biological assay that measures antibodies,…
Q: A lawn of bacteria is placed on the agar surface of a plate and then exposed to UV for 5 minutes.…
A: Bacteria come under the category of single celled, tiny creatures that can be found in all the…
Q: What is the role of the following in the alkaline plasmid screen? G buffer (Cell Suspension…
A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…
Q: A technique used to determine the tertiary structure of a protein is— high-performance liquid…
A: Proteins are macromolecules formed by amino acids. They are large size molecules, polymers of…
Q: DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette…
A: DNA is the nucleic acid and it absorbs the UV light with maximum absorbance at 260 nm wavelength due…
Q: Following electrophoresis of the two pGlo mini-preparation samples (both digested and undigested),…
A: Electrophoresis is a technique wherein a sample of nucleic acids or proteins can be separated on the…
Q: Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do?…
A: Bradford assay is the process of Spectroscopic determination of concentration of total protein in a…
Q: If your ligation and transformation reactions were successful, what will you see on the…
A: Selectable markers or genetic markers are the tools used to identify host cells that have…
Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…
A: The last two years have been extremely difficult because you can still learn and understand the…
Q: a. Preparing/determining the concentration of the agarose gel- b. Loading the samples- c. Choosing…
A: a. Preparing/determining the concentration of the agarose gel- The more the concentration of the…
Q: Complete the following table summarising the neutral red assay data from the group following…
A: Cell Viability Assays -- It is a measure of the proportion of healthy , live cells with in a…
Q: The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text…
A: Osmolarity is defined as the number of moles of solute present in 1 litre of solution. When a…
Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.
A:
Q: Besides agarosc gel electrophoresis, there is also macromolccules. Differentiate both these methods.…
A: Agarose gel electrophoresis and SDS-PAGE are techniques for separation of macromolecules.
Q: Which test require a straight line inoculation? Choose all that apply. A) Bile esculinase B) NaCl…
A: Inoculation is a form of immunisation that involves the introduction of an infectious material onto…
Q: PCR is probably a less sensitive assay than Southern blotting (this relates to the sample size…
A: PCR (polymerase chain reaction is a technique through which a DNA segment is copied to a million…
Q: O C) Generally not required for well-established E. ull cultures O (D) Both A and B are correct O A…
A: Few important points to know about culture media of bacteria ,cell extract and SDS page: For culture…
Q: Whole blood collected for DNA-typing purposes mustbe placed in a vacuum containing the…
A: Every individual has unique DNA, just like fingerprints. The smallest coding portion of DNA is…
Q: G CAT III || || || IIL| | III|| I | | II || | ||| || |L ||
A: Sanger sequencing, additionally called the “chain termination method”, maybe a technique for…
Q: 9. Which of the following colored-top tubes can beused for trace elements assay? O A. K2EDTA…
A: Disclaimer: There are multiple questions here but has failed to specify which question needs to be…
Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS)…
A: Deoxyribonucleic acid is a polymer made up of two polynucleotide chains that coil around one another…
Q: If the stop nucleotides were not added prior to loading the samples in the gel, what would the…
A: Gel electrophoresis is a molecular biology technique used to separate the fragments of nucleotide…
Q: Which of the given statements is correct in the context of visualizing DNA molecules separated by…
A: In Agarose Gel electrophoresis DNA fragments are separated under size. The negatively charged DNA…
Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…
A: A negative control is a control group in an experiment that uses a treatment that will not produce…
Q: ein isolation an Technique Isolation techniques Centrifugation Salting out Isoelectric precipitation…
A: The purification of a protein is important for determining its structure and characterization of its…
Q: When a sample presents some matrix interference, which of the ff. are carried out? Dilute sample to…
A: shift in analytical results caused by one or more specific constituents in the matrix, generally…
Q: The total number of cells in a culture is counted using the trypan blue exclusion assay and is found…
A: Dilutions are used in microbiology to reduce bacterial concentrations to a needed concentration for…
Q: Which of the following is FALSE about DNA fingerprinting? OA DNA is negatively charged so it moves…
A: DNA Fingerprinting or DNA Profiling- TECHNIQUE- For this, only a small amount of DNA like blood,…
Q: Procedure Add 50 of ul the Mix Sml Take 3 ml Top up to 50ml. overnight culture with 45 ml LB -…
A:
Q: Calculate the scan time for a GRE with TR = 30 msec, NEX = 2, Ny = 256, for (a) a single slice and…
A: The gradient-echo (GRE) pulse sequence is also known as gradient recalled echo. The main purpose…
Q: Biuret reagent used in protein assay consists of I. Biuret II. BSA III. Copper (II) sulfate IV.…
A: The biuret test checks for the presence of peptide bonds in the test solution. A peptide bond links…
Q: 5ml of 1 x 10° cells/ ml cell suspension is required to be prepared for seeding on the cell culture…
A: The hemocytometer is a counting chamber or slide used for the purpose of cell counting. It was…
Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…
A: Biochemical test are different types of test carried out to find the nature and identify unknown…
Q: SDS is required to unfold proteins during SDS-PAGE electrophoresis and it is included in both the…
A: SDS-PAGE is known as sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SDS-PAGE protein…
Q: Congo red is a red acidic stain and methylene blue is a l a bacterial smear with a mixture of eosin…
A: Eosin Methylene Blue (EMB) agar is both selective and differential culture medium. It is a selective…
Q: The functions of the loading dye are: (select all that apply) | to prevent sample degradation as gel…
A: It is a mixture of certain chemicals and the chemical composition differ from dye to dye depending…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- I need to prepare a standard calibration curve for gamma globulin. absorbances on Y and mg of standard protein per assay on X. used 0.1mg/ml gamma stock for tubes 2-6. (Water (ml), gamma (ml), Abosrbance)--> (.036, .004, .290) (.036, .008, .358) (.024, .016, .341) (.016, .024, .520) (.008, .032, .597) - What is the math and how do you get the standard curve?After performing the manual Albumin assay, you get the absorbance value of 0.205 for a 4.5 g/dL albumin standard and 0.114 for control A. What is the calculated value of the control? (just write a number with one decimal, the units will be mg/dL)4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?
- You will use a Calf Thymus DNA standard stock solution (1 mg/ml) to prepare a standard curve. Calculate volume of stock solution in ul, needed to prepare 10 ug/ml dilution (final volume 1.0 ml).The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?
- 1. In the preparation of the standard curve for protein analysis, 50 mnBSA (bovine serum albumin) dissolved in H2O to a final volume of 5mL was used as stock solution. What is the weight of BSA in 0.1 mL ofstock solution? In 0.2 mL? 2. The above mentioned aliquots (#9) which were diluted with enoughwater to a final volume of 1 mL were assayed colorimetrically andyielded the following absorbance readings:mL stock mL H2O Absorbance0.2 0.8 0.1000.4 0.6 0.2000.6 0.4 0.3000.8 0.2 0.4001.0 0.0 0.500 i. Tabulate mg BSA vs. absorbance readingii. Draw the curve where mg BSA is on x-axis and absorbance ony-axis (use graphing paper).iii. Calculate the slope of the curveiv. If a sample solution has an absorbance of 0.332, determinemg protein of the sample.After performing a plate and liquid lysate, it was found that the plate lysate achieved the common titre range of 1010-1011 pfu/ml whereas the liquid lysate achieved a range of 108 PFU/mL; what is the reason for the lower titre in different methodologies? How can we troubleshoot this issue in the future?You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.
- You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose. Please answer with explanation ASAP. I will really upvote. ThanksTotal protein: Do you think that refractive index could be used as a regular method for serum protein determinations? If yes, what limitations does it have. If not, why not? What are the major interferences in the biuret method? Refractometry method?A 250 mL IV solution contains 25,000 units of heparin. The desired dosage is 8 units/kg/hr. The patient weighs 220 Ibs. The drop factor is 60 gtts/mL.. How many units of heparin are in 5 mL of solution? (Hint: how many are in 1 mL? Now, how many are in 5?)