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Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm.
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- At the completion of the experiment, a student measures the solvent front to be 55 mm from the baseline. If a component in the sample being analyzed generates a spot whose diameter spans from 34 to 36 mm from the baseline, calculate the Rf value for that component.Calculate the RF value for a spot in a TLC experiment if the solvent move 13.5 cm and the spot move 9.1 cm from the originWhat are the retention times and areas of all the attached peaks?
- What error is introduced into the determination of an Rf value if the top is left off the developing chamber?Does the amount of radiation (cpm) increase or decrease as the distance between the sorce and the sensor increases? ExplainCalculate the Rf value for a spot in a TLC experiment if the solvent moved 14.9 cm and the spot moved 9.5 cm from the origin.
- If a spot travels 2.3 cm while the mobile phase travels 6.6 cm, what is the Rf value of the substance in the spot? What is the mobile phase used and stationary phase used in this lab?a pigment in the thin layer chromatography traveled beyond or farther than the solvent front. Therefore, the Rf value of that certain pigment is greater than 1. What does this mean to the experiment?Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the following in your drawing: Solvent front Origin Mobile phase Stationary phase Distances needed to measure Rf value for one of the spots