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- a. Briefly explain why internal standardization method is useful inanalytical chemistry?b. Why does a response factor of an instrument’s detector need to becalculated?What is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum ).which of the folowing(s) is/are true ? 1 split ratio in GC is a ratio of sample passed to column to amount of mobile phashe 2 column resolution decreases with increasing column lenght 3 decreasing the tube diameter ,increases the resolution
- How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your own words. Answer should be to the point (5-6 lines maximum).What is the experimental purpose of constructing a standard curve? a. To achieve an appropriate dilution factor for SDS-PAGE b. To verify the concentrations of the Bradford reagent within known parameters c. To produce a linear equation to estimate analyte concentration d. To verify that the serial dilution was properly conductedA 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to 100.0 mL in a volumetric flask. Aliquots of the sample solution were treated with a lead complexing reagent and water as follows: Solution 1: 10.0 ml blood sample + 20.0 mL complexing agent + 30.0 mL H20. Solution 2: 10.0 ml blood sample + 20.0 mL complexing agent + 26.0 mL H20 + 4.00 mL of 78 ppb Pb2+ standard. The resulting solutions were analyzed by UV/Vis at 375 nm. Absorbance for solution 1 = 0.155 and for solution 2 = 0.216. Calculate the concentration of lead (ppb) in the original sample.
- 21. (What exactly is meant by potentiometric methods? What are they even?)The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown Abs. BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.600 0.635 Abs. BIg 0 0.080 0.230 0.460 0.520 0.410 0.510 0.700 Calculate the concentration of the unknown protein in mg/mlDetermine the relationship between the observed/apparent value (EX) VERSUS that of the true value (ET) for the quantity being sought by writing either <, >, or = on the space provided Determination of the true concentration (ppm Fe) in a water sample using the iron-phenanthroline method. Dilution factor was not taken into account. EX _____ 1ET
- Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary phase particle size is reduced?a- Separation is essential in chemical analysis especially when we are dealing with complexmixture of chemical species. Name 1 separation technique based on basis of operation asbelow. Basis of operation Techniquei) Sizeii) Mass and densityiii) Change in physical stateChemistry Column 25 m length and 0.2 mm I.D. has efficiency is 100,000 theoretical plates; Flow rate is 1 mL/min. Calculate the peak width for component with retention factor 2. Which data acquisition sampling rate should be used for proper quantification of this component? Explain. (For accurate integration peak should have at least
