The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown Abs. BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.600 0.635 Abs. BIg 0 0.080 0.230 0.460 0.520 0.410 0.510 0.700 Calculate the concentration of the unknown protein in mg/ml
The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown Abs. BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.600 0.635 Abs. BIg 0 0.080 0.230 0.460 0.520 0.410 0.510 0.700 Calculate the concentration of the unknown protein in mg/ml
Chapter24: Introduction To Spectrochemical Methods
Section: Chapter Questions
Problem 24.28QAP
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The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples
The following results was obtained:
Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method
|
Protein (mg/ml) |
0.00 |
0.125 |
0.25 |
0.50 |
0.75 |
1.00 |
1.50 |
2.00 |
Unknown |
|
Abs. BSA |
0 |
0.130 |
0.350 |
0.620 |
0.870 |
1.120 |
1.400 |
1.600 |
0.635 |
|
Abs. BIg |
0 |
0.080 |
0.230 |
0.460 |
0.520 |
0.410 |
0.510 |
0.700 |
Calculate the concentration of the unknown protein in mg/ml
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