Can someone check my answers and see if they are correct? © Macmillan Learnglutamate and lysineaspartate and valineserine and alanineglutamateaspartatealanine▼arginine and methionineglycine and leucinemethionineleucine O Macmillan LearningMixtures of amino acids can be analyzed by first separating the mixture into its components throughion-exchange chromatography.Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different ratesbecause of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column andpositively charged functional groups on the amino acids, and (2) aggregation of nonpolar amino acid side chains with thehydrophobic backbone of the polystyrene resin.Note that the ionic attraction is more important than hydrophobicity for this column media.For each pair of amino acids, identify which will be eluted first from a cation-exchange column using a pH 7.0 buffer.

The stationary phase (-SO3-) is negatively charged (Cation-exchange chromatography). Since the…

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Chemistry

Can someone check my answers and see if they are correct?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.19QAP

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In the universe, there is matter, energy, and void space. The universe contains energy and void space. The definition of matter can be considered as something which possesses mass and consumes space. Chemistry is that branch of science which deals with the study of matter. Under chemistry, nature, composition, and the changes occurring to matter are discussed.

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Can someone check my answers and see if they are correct?

© Macmillan Learn
glutamate and lysine
aspartate and valine
serine and alanine
glutamate
aspartate
alanine
▼
arginine and methionine
glycine and leucine
methionine
leucine

O Macmillan Learning
Mixtures of amino acids can be analyzed by first separating the mixture into its components through
ion-exchange chromatography.
Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different rates
because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and
positively charged functional groups on the amino acids, and (2) aggregation of nonpolar amino acid side chains with the
hydrophobic backbone of the polystyrene resin.
Note that the ionic attraction is more important than hydrophobicity for this column media.
For each pair of amino acids, identify which will be eluted first from a cation-exchange column using a pH 7.0 buffer.

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