Cell growth kinetics A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is monitored with the following results. Time (days) Cell concentration (cells.ml² x 10°) 0.0 00.2 0.45 0.52 0.65 0.81 0.5 1.0 1.5 1.22 2.0 1.77 2.5 2.13 3.0 3.55 3.5 4.02 4.0 3.77 4.5 2.20 (a) Determine the specific growth rate during the growth phase. (b) What is the culture doubling time?

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Cell growth kinetics
A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is
monitored with the following results.
Time (days)
Cell concentration (cells.ml* x 10°)
0.0
0.45
00.2
0.52
0.5
0.65
1.0
0.81
1.5
1.22
2.0
1.77
2.5
2.13
3.0
3.55
3.5
4.02
4.0
3.77
4.5
2.20
(a) Determine the specific growth rate during the growth phase.
(b) What is the culture doubling time?
Transcribed Image Text:Cell growth kinetics A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is monitored with the following results. Time (days) Cell concentration (cells.ml* x 10°) 0.0 0.45 00.2 0.52 0.5 0.65 1.0 0.81 1.5 1.22 2.0 1.77 2.5 2.13 3.0 3.55 3.5 4.02 4.0 3.77 4.5 2.20 (a) Determine the specific growth rate during the growth phase. (b) What is the culture doubling time?
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