Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain of Escherichia coli (E. coli). b. Estimate the doubling time for this strain of E. Coli. Clearly show how you estimated this value from the empirical data presented.

Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain of Escherichia coli (E. coli). b. Estimate the doubling time for this strain of E. Coli. Clearly show how you estimated this value from the empirical data presented.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter14: Formula Method

Section: Chapter Questions

Problem 40SST

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Time point (min)	Absorbance of culture at 660nm	Approximate cell concentration	Approximate # cells in 1mL extract
0	0.298	1.49 x 10⁸ cells/mL	1.49 x 10⁸ cells
10	0.316	1.58 x 10⁸ cells/mL	1.58 x 10⁸ cells
20	0.374	1.87 x 10⁸ cells/mL	1.87 x 10⁸ cells
30	0.429	2.145 x 10⁸ cells/mL	2.145 x 10⁸ cells
40	0.512	2.56 x 10⁸ cells/mL	2.56 x 10⁸ cells
50	0.544	2.72 x 10⁸ cells/mL	2.72 x 10⁸ cells
60	0.607	3.035 x 10⁸ cells/mL	3.035 x 10⁸ cells