Choosing the proper buffer solution In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer. Buffer solutions                                                               pKa   Acetate buffer                                                                  4.73 Tris- (hydroxymethy) aminomethane                                 8.08 Phosphate buffer                                                               7.20 2. Preparation of the chosen buffer system Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter13: Dimensional Analysis/units Conversion
Section: Chapter Questions
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Choice and Preparation of a Buffer System
1. Choosing the proper buffer solution

In Protein Precipitation, two liters of 5mM buffer solution with pH 5.2 is needed in the isolation of albumin. Which among the following buffer solution is best fitted for said purpose? Justify your answer.
Buffer solutions                                                               pKa
  Acetate buffer                                                                  4.73
Tris- (hydroxymethy) aminomethane                                 8.08
Phosphate buffer                                                               7.20

2. Preparation of the chosen buffer system

Calculate and measure the amounts (in grams if solid and in mL if liquid) of weak acid and conjugate base needed to be able to prepare the chosen buffer system in part A above. Express your answer in useful units (that is, prepare it from practical amounts or concentrations of starting materials).

 

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