1. Erythrose is an aldoterose. Describe the result when it is subjected to each of the following tests:
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topic: ISOLATION AND CHARACTERIZATION OF CARBOHYDRATES
1. Erythrose is an aldoterose. Describe the result when it is subjected to each of the following tests:
a. Molisch’s Test
b. Barfoed’s Test
c. Benedict’s Test
d. Seliwanoff’s Test
2. Illustrate the reaction and identify the principle involved in the Nelson test.
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- Answer the following: A. Define reducing sugars and their properties B. Among the given tests such as Benedict, Iodine and Reagent Test, which is most highly applicable in biomedical field? Explain.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Seliwanoff’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Suggest how the weak points of the Molisch test for carbohydrates can be strengthened by comparing it to other carbohydrate tests
- Topic: Reducing and Non Reducing Carbohydrates Among the Benedict's, Reagent, and Iodine tests, which is most highly applicable in biomedical field? ExplainState the principle that underlies the following biochemical tests: a. Voges-Proskauer testBased on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Fehling’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)
- Explain the reason for using bile as a selective agent and the basis for the preliminary biochemical tests.Outline a method for a fully quantitative test for reducing sugars. Please keep it simplea) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sample