Circle the correct word or phrase in each bracketed region to complete a correct statement. Crispr/Cas9 genome editing depends on a(n) [tRNA / guide RNA / snRNA / DNA primer] that directs Cas9 [endonuclease / ribonuclease / protease / exonuclease] to specific sites in the genome by [binding to the major groove / PAM site recognition / binding via base complementarity]. Cas9 creates [double-stranded DNA breaks / depurination / PAM site deletion / CpG methylation] that can be repaired by [nonhomologous end joining / ligation / homology directed repair / CpG methylation] to create precise edits or [nonhomologous end joining / ligation / homology directed repair / CpG methylation] to create insertions & deletions

Q: Choose one product of recominbinant DNA technology and write the steps involved in the production of…

A: The Flavr Savr was the first commercially produced genetically modified food to receive a human…

Q: Please choose the correct answer. Initial DNA nucleotides are added by reverse transcriptase from…

A: Reverse transcriptase is RNA dependent DNA polymerase. It transcribes ssRNA into DNA.

Q: Explain your experimental approach if you must correct the genetic defect phenylketonuria, which is…

A: Phenylketonuria (commonly known as PKU) is an inherited disorder that increases the levels of a…

Q: The red line indicates where a radioactively labeled probe hybridizes. Three samples of the genomic…

A: P                      B                         PB…

Q: Tm value, the GC-content of the selected DNA sequence and the absolute nucleotide composition of the…

A: DNA stands for deoxyribonucleic acid and it consists of 4 types of nitrogen bases, adenine, guanine,…

Q: Can you select answers in each bracket? CRISPR-Cas9 is a system for directly changing the sequence…

A: CRISPR is a group of DNA sequences present in the genomes of bacteria and archaea, which are…

Q: ABOUT Phenylketonuria Explain Potential technical issues and limitations of PCR technology are…

A: Phenylketonuria (PKU) is a metabolic disorder in which the amino acid phenylalanine is not properly…

Q: Describe post-transcriptional modifications that occur in mRNA and tRNA. Please type your answer.…

A: mRNA is known as massanger RNA. About 3-5% of the cellular RNA are of m-RNA type. It is linear in…

Q: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting…

A: The recombinant pharmaceuticals are the products of recombinant DNA technology like insulin,…

Q: Below is a DNA strand.  We will call this the original strand, and you will transcribe and translate…

A:

Q: Which of the following is a transversion mutation Select 4 correct answer(s) OA --> T OC--> T C -->…

A: Point mutations The mutations that occur at single base nucleotides are called a base or point…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: DNA is a nucleic acid formed of two strands of nucleotides. The nucleotides have nitrogen bases that…

Q: Using the chart posted to the left of the microwave in lab, what is the 3. restriction recognition…

A: Step 1 In 1963, Arber discovered that Escherichia coli is able to protect itself from the attack of…

Q: The following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and…

A: Restriction enzymes play a major role in recombinant DNA technology as they can recognise a specific…

Q: Complete each of the following statements by selecting from the bank of terms below. a. tRNA b.…

A: 81)Redundancy 82)Universal  83)Elongation  84)Promotor 85)RNA processing  86)snRNA 87)tRNA

Q: In your earlier research for this case study, you have discovered that nucleotides 1591-1593 (in the…

A: Cystic fibrosis (CF) is a genetic disorder characterized by mutations in the cystic fibrosis…

Q: The code for a fully functional protein is actually coming from an mRNA transcript that has…

A: Protein synthesis or translation process involve at least three steps- In the first step there is a…

Q: In organism that is mutant for components of the non-homologous end-joining NHEJ repair pathway…

A: CRISPR gene editing is a molecular biology genetic engineering tool for altering the genomes of…

Q: could you write about 4-5 sentences explaining what CRISPR is

A: Humans started manipulating genome initially by controlling breeding and through selection of…

Q: Please help transfer CAT AGG GAG to mRNA

A: Answer. DNA replication is a process that takes place inside the nucleus of the cell. During…

Q: Which of the following statements about CRISPR are accurate? Select all that apply. O CRISPR…

A: The most accurate statements regarding CRISPR are - a, c and e.

Q: How does the sequence of the primary transcript resemble the sequence of the gene encoding it?…

A: The primary mRNA transcript is practical as soon as it's far synthesized. This is visible when…

Q: You want to edit the cystic fibrosis gene using the CRISPR-cas9 system. In order to target your gene…

A: CRISPR/cas is a part of adaptive immune system of bacteria. It can be used in gene editing.

Q: Identify which mutagen is described by the following statement. Results to the deamination of…

A: Mutagens are substances that induces mutation. Chemical mutagens are certain chemicals that induces…

Q: Give the SIGNIFICANT difference between the terms provided in the table below. NO NEED for complete…

A: Somatic mutations occur in a single body cell and it cannot be inherited whereas germline mutation…

Q: Explain how the mRNA vaccine work and relate to protein synthesis  (Moderna and Pfizer vaccines are…

A: mRNA (messenger RNA) is a single-stranded molecule found in all of our cells. It transports the…

Q: Below is a set of sequence reads from an individual aligned to a reference sequence. AGACACTCCACC…

A: Homozygous SNPs - has multiple possible alleles - 1 Heterozygous SNPs - has specific insertions or…

Q: CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene…

A: Design a set of targetting sequences using algorithms. For successful genome editing, the major…

Q: The following bacterial DNA genome is composed of three sequences: TGGCGGT / ACGTTAC / GGATAGG Using…

A: Clustered regularly interspaced short palindromic repeats or CRISPR is a family of nucleotide…

Q: Which of the following statement(s) is/are true. You can select multiple options. Select all that…

A: Insertion or deletion of 3 or more nucleotides does not cause frame shift mutation. Frame shift…

Q: Select all true statements regarding CRISPR: A. CRISPR stands for Clustered regularly interspaced…

A: Genome editing refers to a set of techniques that enable scientists to alter an organism's DNA.…

Q: A transposable element or sequence of DNA that can move within the genome Group of answer choices…

A: Transposable elements or sequences of DNA that can move within the genome are also called jumping…

Q: Where did the Crispr system first originate and what is its purpose in nature? What is the advantage…

A: We are answering first 4 questions. For rest of questions pls repost.

Q: You want to determine the transcriptomic response to heat shock in [Choose ] a normal cell line.…

A: Transcriptomic is the study of total RNA present at a particular time in a particular cell. To…

Q: Identify which mutagen is described by the following statement. Inserts between adjacent bases in…

A: Certain chemical mutagen can insert between adjacent base pairs of DNA molecule e.g., proflavine…

Q: ive the SIGNIFICANT difference between the terms provided in the table below.

A: Somatic Mutation   Germline Mutation     It occurs in the non-germline cells i.e. except…

Q: Your next task is to study Ebola virus (EboV) sequences of different strains. You have received…

A: Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to…

Q: Transcription

A: RNA polymerase synthesizes an RNA transcript complementary to the DNA template strand in the 5' to…

Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…

A: A- recombinant plasmid  B- developmental genetics

Q: 39. Answer and explain

A: Mutagens are agents which cause mutation. Mutation is the change in the sequence of nucleotides in…

Q: Complete each sentence with the appropriate term or phrase. (Each box can be used only once, and not…

A:

Q: Attached is a cartoon model of the CRISPR system, with 3 critical components labeled.  A. What is…

A: CRISPR-Cas9 has recently become a popular set of tools for genetic engineering. By targeting…

Q: Although CRISPR uses some of the same viral vectors as "gene therapy," which was pioneered in the…

A: Gene therapy is the process of changing the genes in the body's cells in order to treat or prevent…

Q: Identify which mutagen is described by the following statement. Results to the deamination of…

A: NOTE: The options are numbered as 1, 2, 3, 4, 5, and 6.  DNA(deoxyribonucleic acid) is the basic…

Q: Following replication of the viral RNA, the mRNA coding for the Spike protein is produced. The part…

A: Codon is the sequence that is made of 3 consecutive amino acids that code for a specific amino acid.…

Q: Name the components and the function of each of a CRISPR/Cas9 genetic editing system

A: A defense mechanism that is present in many bacteria and archaea is termed as CRSPR/Cas system. This…

Q: Provide the SIGNIFICANT difference between the terms provided in the table below.

A: 1. Missense mutation is the change in the the sense codon at which place another sense codon is…

Q: Write TRUE or FALSE. If false, write the word/s that make(s) the statement incorrect. 1. In RNAi,…

A: Introduction :  Cancer is a disease which occurs due to the uncontrollable growth of certain body…

Q: The code for a fully functional protein is actually coming from an mRNA transcript that has…

A: Conversation of non functional mRNA to functional mRNA is done in many steps.. 1. A…

Q: According to the genome structure, classify   SARS-CoV-2  a. ss DNA b. ds DNA c. ss RNA d. ds RNA

A: A virus is a minuscule infectious agent that multiplies solely within an organism's live cells. From…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.