Identify which mutagen is described by the following statement. Results to the deamination of nitrogenous bases. I Select ] A [ Select] intercalating agent UV ray nitrous acid base analogue aflatoxin X-ray - Previous
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- Explain with help of diagrams the mutagenic effects on DNA of following mutagens? Below is an example i. Ultraviolet light ii. X- raysiii. Base analogsiv. Hydroxylaminev. nitrous acidvi. ethylmethane sulfonateDefine the following terms:a. nonreplicative transpositionb. replicative transpositionc. composite transposond. retrotransposone. insertional elementList three possible uses of site-directed mutagenesis
- Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfateTitled “Techniques utilized to generate a genetically engineered organism and confirm gene disruption.” a. In the table, indicate with an "x" if the reagent or technique applies to DNA, RNA, and/or protein. You can have more than one "x" in each row or none at all.Please verify if correct or not. If incorrect, please match descriptions with the correct mutation.
- Record the type of mutation obtained for the mutagens. Mutagen Alkylator. Was it point, addition, or deletion Mutagen ultravioelt. Was it point, additon, or deletion.Choose the combination of answers that most accurately completes the statement.Which of the following sequences, when combined with its complement, would be clipped by a restriction endonuclease? a. ATCGATCGTAGCTA c. GAATTC b. AAGCTTCGAA d. ACCATTGGAYour mutagenized sample showed 523 CFU after plating 100uL of media on the 10^3 dilution plate. The control, unmutagenized, sample showed 417 CFU after plating 100uL of media on the 10^8 dilution plate. Calculate the % survivorship for this mutagenesis experiment.
- Ehat primer sets could be amplify the following DNA sequence? AATACGTCGCATGGggatccttttttatgcatgFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action 2-aminopurine Acridine orange ProflavineFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Heat 5-bromouracil Ethidium bromide