A pure bacterial culture of unknown concentration was diluted to determine theconcentration of viable bacteria in the original culture. Serial dilutions were performed as2.diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates wereinoculated with 100 µl from the last three dilution tubes.d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies wouldyou expect to form on the plate after incubation?e. If the original culture had a volume of 50ml, what was the total number of viablebacteria in the 50 ml of the original culture?232403Number of colonies

2d) In the initial diagram given, the number of colonies from tube 4, that were formed on the plate…

d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture?

d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture?

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 4CR

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Question

A pure bacterial culture of unknown concentration was diluted to determine the
concentration of viable bacteria in the original culture. Serial dilutions were performed as
2.
diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates were
inoculated with 100 µl from the last three dilution tubes.
d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would
you expect to form on the plate after incubation?
e. If the original culture had a volume of 50ml, what was the total number of viable
bacteria in the 50 ml of the original culture?
2
3
240
3
Number of colonies

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2d) 30 colonies, if the plated volume is 1ml from tube 4 2e) Original viable cell no. 24 x 10^5

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