Q: How might you isolate a morphologically unique bacteriumpresent in an enrichment culture in…
A: A culture or growth medium is a solid, liquid, or semi-solid designed to support the growth of a…
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…
A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…
Q: What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line…
A: An isolated colony represents a pure source of an organism from which a pure culture can be started.
Q: explain how the following genotypic and phenotypic methods of microbe identification is done. Use…
A: The biochemical tests are the tests that are used to identify various types of microbial species.…
Q: Why is the level of contamination measured as number of colonies rather than size of colonies?
A: Microbiological contamination refers to the presence of unwanted or accidental introduction of…
Q: In what instances would the pour plate method be more appropriate than the spread plat method?
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Q: Gram stain of Nodule Suspension Describe the morphology of the crushed nodules under microscopes…
A: The bacteria are stained with the gram stain and can be divided into two groups on the basis of the…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…
A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: Identify one culture-based and one non-culture-based method used for analyzing bacterial growth.
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Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…
A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…
Q: If you had cultures of B. subtilis and E. aerogenes and forgot to label them, which of the following…
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Q: Discuss the advantages of the Gram stain procedure in microbiology.
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Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…
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Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…
A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…
Q: Describe the manner of inoculation for different types of tube media.
A: Microbiology is the study of microorganisms and associated ideas. Microbiology has gone a long way…
Q: what is the diffrence between Aseptic technique and sterile technique
A: Aseptic technique Also called as asepsis. It means absence of germs such as bacteria, viruses, and…
Q: You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate…
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Q: Outline the steps in gram staining
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Q: Which technique(s) allow us to obtain a single, pure colony?
A: Isolation of pure colony - Pure culture, in biology, a laboratory culture containing one species of…
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Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.
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Q: Give at least two other examples of samples that are best prepared using Smear Preparation…
A: Smear preparation technique: it is used to fix the samples onto the slides.
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A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
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Q: The main of objective of using 13-Line streaking method is to get:
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Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…
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Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
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Q: how to find out the test is differential or selective in the microbiology lab test?
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Q: What are the advantages and disadvantages of using the Slide Culture technique?
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Q: Why is it so important to develop tests to ID bacteria
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Q: Give 2 different reasons that scientists might need to isolate a pure culture.
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Q: Are there technologies that can help make Bacterial Culture and Sensitivity done faster?
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Describe conventional techniques used to isolate single colonies and establish a pure culture
Step by step
Solved in 2 steps
- Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture can be stored.What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?
- What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?How does the agar dilution method differ from streaking toobtain isolated colonies?discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
- For the pour plate method, state which plate will be chosen for colony isolation and why? From the chosen plate, indicate (circle) the colonies for both bacterial species that will be selected and transferred to new nutrient agars as a pure culture.What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?Explain the significance of aseptic culture technique in growing microorganisms.