Describe the role of sodium and phosphomolybdate reagent in Folin Wu method.
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1. Describe the role of sodium and phosphomolybdate reagent in Folin Wu method.
2. Describe the accuracy and specificity of Nelson-Somogyi method
3. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.
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- 1. Describe the accuracy and specificity of Nelson-Somogyi method 2. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.1. If you are a medical technologist, which of the four methods of glucose determination would you employ? why? 2. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer. 3.Describe the accuracy and specificity of Nelson-Somogyi method.1.State the principle that underlies the following biochemical tests: a) Methyl red testb) Voges-Proskauer test 2. how does oxidative phosphorylation differ from substrate-level phosphorylation?
- Match the chemical reaction to the visual effect of the product/biochemical test description 1. This assay's color change relies on an initial reduction reaction resulting in a black precipitate if the organisms is "positive" 2. The visual production of gas from this reaction indicates this organism can break down toxic byproducts of aerobic respiration. 3. This assay relies on a pH indicator (phenol red) for a color change. An organism that was "positive" for the certain enzyme in this assay, would result in a pink color change. 4. If an organism was incubated in simple broth containing a phenol red pH indicator and a sugar source, and the broth turned yellow following bacterial growth, that would be evidence of this reaction occurring. 5. The reaction representing this assay, works by producing a red colored product upon addition of a reagent, which tells us the organism possesses the enzyme required to break down a particular amino acid.Compare and contrast the glucose oxidase and hexokinase methods of glucose determination and relate these procedures to the clinitest method. Please Type Thanks Don't copy any where33. Which of the following is an emulsifying agent? a. bile salt b. NarCOs c. both a and b d. neither a nor b 34. What reaction occurs during saponification of triacylglycerol? a. hydrolysis b. condensation c. both a and b d. neither a nor b 36. What was added to the sample prior to heating in acrolein test? a. KHSO4 b. ethanol c. both a and b d. neither a nor b 37. Which of the following is a general test for lipids? a. solubility test b. Huble's test c. both a and b d. neither a nor b 38. Which test can distinguish between sesame oil and corn oil? a. Burchard test b. Baudouin test c. Sudan IV test d. Dichromate test 39. What solvent would be miscible with coconut oil? a. chloroform b. water c. both a and b d. neither a nor b 40. Which of the following is a product of saponification of fatty acids? a. glycerol b. NaOH c. both a and b d. neither a nor b
- 1. What type of reaction occurred when the samples (enumerated) reacted with the Molisch reagent? Give the chemical equation for each.a. Glucoseb. Sucrosec. Fructosed. Lactosee. Galactosef. Ribose 2. Explain the mechanism behind the test in simple terms. 3. What are the important products of the reaction of each sample?1. in cherry crandall method for lipase analysis what is the substrate used? 2. which of the following is the reason why hemolysis shoule be avoided? a. enzymes have lower concentration in cells thus hemolysis increases levels in serum or plasma b. hemolysis does not affect plasma and serum enzyme levels c. enzymes are specific to the target issue d. none of these e. enzymes have higher concentration in cells thus hemlysis increases level in serum or plasma 3. increasing temperature usually increases the rate of a chemical reaction by increasing the movement of molecules. The rate of denaturation increases as the temperature increases and is usually significant at 40C to 50C a. both statement are false b. both statements are true c. first statement is correct, second statement is false d. second statement is true, first statement is falseIn lactose fermentation test what does V means? Please help
- GIVE A DETAILED AND COMPLETE DESCRIPTION FOR THE FOLLOWING: 1. What are the culture media used in the following tests? — indole production test (indole production from tryptophan) — lysine decarboxylase test 2. What are the expected results in the following tests? — indole production test (indole production from tryptophan) — lysine decarboxylase test1which test is used to differentiate between fermentative and oxidative metabolism?describe the different result that may be observed with this test. 2.Which of the five hydrolysis tests (starch, casein, triglycerides, tryptophan, and urea) would a positive test result be expected for the etiologic agent of acne, Propionibacterium acnes? Explain1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. (5 points) 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. (5 points) 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. ( 5 points) 4. Discuss why human blood plasma will not always yield reliable results. (5 points)