Control - Rat liver Suspected mutagen Rat liver extract extract Salmonella- strain (requires histidine) -Salmonella strain (requires histidine) Plate the mixtures onto petri plates that lack histidine. Incubate overnight to allow bacterial growth. A large number of colonies suggests that the suspected mutagen causes mutation. FIGURE 19.15 The Ames test for mutagenicity.
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What is the purpose of the rat liver extract in this procedure?
Mutations refer to the random changes in the genome of the organisms.
The chemicals or any other substances that can induce mutations are called mutants.
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