For each of the following items, describe what it is explain the necessity for each in cloning plasmids that are used for the expression (production) of protein. what would happen if this element was omitted? The Multiple Cloning Site (MCS) Origin of replication – (AmpR) antibiotic resistance gene – T7 promoter - T7 termination site- lac I gene

For each of the following items, describe what it is explain the necessity for each in cloning plasmids that are used for the expression (production) of protein. what would happen if this element was omitted? The Multiple Cloning Site (MCS) Origin of replication – (AmpR) antibiotic resistance gene – T7 promoter - T7 termination site- lac I gene

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 5P: Molecules of DNA Polymerase III per Cell vs. Growth Rate It is estimated that there are 40 molecules...

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HOW THE RECOMBINANT PROTEIN COULD BE EXPRESSED IN E. COLI 1. STRATEGIES TO IMPROVE THE PROTEIN EXPRESSION
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