5. You are imprisoned in a biochemistry lab by an ogre (one of Shrek's kids) who wants you to purify a specific protein from E. coli. You know the protein has a pl of 6.0, and besides lots of buffers, you also have access to DEAE ion exchange resin. What will you do and at what pH will you do it? 6. If the only ion exchange resin available was carboxymethyl (CM) how would you modify the protocol you described in question 5?

5. You are imprisoned in a biochemistry lab by an ogre (one of Shrek's kids) who wants you to purify a specific protein from E. coli. You know the protein has a pl of 6.0, and besides lots of buffers, you also have access to DEAE ion exchange resin. What will you do and at what pH will you do it? 6. If the only ion exchange resin available was carboxymethyl (CM) how would you modify the protocol you described in question 5?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter6: Energy, Enzymes, And Biological Reactions

Section: Chapter Questions

Problem 2TYK: The assembly of proteins from amino acids is best described as: a. a conversion of kinetic energy to...

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The assembly of proteins from amino acids is best described as: a. a conversion of kinetic energy to potential energy reaction. b. an entropy reaction. c. a catabolic reaction. d. an anabolic reaction. e. an energy-free reaction.
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