2. Below is data from an HFR time of entry gene mapping experiment. Create a map of the chromosome with all genes labeled and the location and orientations of the oriT sites. Hfr Strain Data orit gal ton azi lac leu oriT azi ton gal trp cys oriT met ade1 arg cys trp 2 4 ortT met ade2 thr leu lac

2. Below is data from an HFR time of entry gene mapping experiment. Create a map of the chromosome with all genes labeled and the location and orientations of the oriT sites. Hfr Strain Data orit gal ton azi lac leu oriT azi ton gal trp cys oriT met ade1 arg cys trp 2 4 ortT met ade2 thr leu lac

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 8TYK

See similar textbooks

Related questions

Q: A bacterial cell in which the F plasmid is integrated into the chromosome is known as an F-…

A: Hfr cell is defined as high-frequency recombination cell is a bacterium which is a conjugative…

Q: Biology Section 12BC / School Year / We. kl197624 All changes saved 8. In Labrador Retrievers, coat…

A: Gene interaction: When there is a change in a single trait by the influence of more than two…

Q: 18. Choose the correct number of DNA molecules created after 6 cycles of PCR. a. 6 b. 16 c. 32 d. 64…

A: Introduction The polymerase chain reaction (PCR) is a widely used method for rapidly making…

Q: An F prime factor: A) O originates from an Hfr strain B) O represents an infrequent, abnormal…

A: Bacteria is the unicellular organisms they are different types of bacteria many lives in different…

Q: 1. Identical twins may show dissimilar phenotypes due to changed methylation patterns of the…

A: * monozygotic twins even they are genetically identical there will be some variation in them the…

Q: 17. The diagram below shows two chromosomes, M and N, before and after a chromosomal mutation. The…

A: Mutation Sudden heritable change in genetic material of an organism is called as mutation. Mutation…

Q: What would be the chromosome number of progeny resulting from the following crosses in wheat (see…

A: Genetics is the study of genes and their functions. Genes are the regions in the DNA that code for…

Q: 1. Below are the genotypes for two individuals. Loci A and B are two different microsatellites. The…

A: Allele is the alternate form of a gene.

Q: For a coding gene, purifying (negative) selection will result in more synonymous (silent) site…

A: Purifying selection is the most predominant form of natural selection or negative selection. As it…

Q: The drawing below depicts a restriction map of a segment of a human chromosome. The cut sites shown…

A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. If you need help with other sub…

Q: What is the expected frequency of the restriction site 5′−GGCC−3′ in a genome? A. Once every 4…

Q: 1. _____________ frequencies are used to obtain a genetic map. A. Transduction B.…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: 6 - Restriction enzymes are used to Amplify the gene of interest D) O Ligase vector and gene of…

A: The restriction enzymes or restriction endonucleases are enzymes that cleave double-stranded DNA…

Q: i. Mutant A has a single base pair substitution with the T/A being replaced with C/G base pair at…

A: The transcription unit has a coding strand and template strand. Out of these two strands, only the…

Q: P1 F1 F2 1. Line 1 with pisatin 3 with pisatin X resistant 1 no pisatin www 2. Line 2 X resistant no…

A: Homozygous The condition in which the sets of gene are same either recessive or dominant.

Q: In mapping experiments, ______ strains are conjugated to F−strains. The distance between two genes…

A: Conjugation refers to the the temporary union of two bacteria or unicellular organisms for the…

Q: A wrinkle-seeded plant (rr) is crossed with a round-seeded plant (Rr). WHAT is the punnet squar for…

A: An allele is a variant form a gene and is present in two forms: dominant and recessive. The dominant…

Q: 4) If 20% of a culture of human cells have a DNA content somewhere between 1&2xs (S-phase= 8 hours)…

A: S phase is synthesis phase during which DNA is replicated when the cell is undergoing mitosis.

Q: 7. Refer to this map to answer the following questions: 20 cm Gene D Gene E Gene F 8 cm a. What is…

A: INTRODUCTION: In the figure we have to calculate, distance between two genes, and probability of no…

Q: 9. What can be inferred about the red gene versus the blue gene in the following diagram? II II…

A: Genetic variation refers to diversity in gene frequencies. Genetic variation can refer to…

Q: . Which of the following statements about STRs is correct? 1. They are highly polymorphic. 2.They…

A: STR stands for Short Tandem Repeat, it is a region of a DNA molecules contains short segment that…

Q: 3. A plasmid was digested with the enzymes BglII, Nsil and XhoI. A single digest was performed with…

A: A plasmid is an extrachromosomal small DNA molecule present in a cell and is also physically…

Q: The photo where there is written "procedure" is the procedure, while the other photo where there is…

A: Linked Genes ---Introduction and Hypothesis --Answer ( a ) When genes are on separate chromosome or…

Q: Which of the following may be detected using a karyotype?a. point mutationsb. a specific genetic…

A: Karyotype refers to a laboratory technique to produce an image of an individual’s chromosomes.

Q: 2. What is the result of crossing over? A. recombination B. mutation C. linkage D. adaptation

A: The field of biology that deals with the study of inheritance are known as genetics. Organisms pass…

Q: 4. Draw a diagram clearly showing how intragenic recombination can take an individual who is cc and…

Q: 6. What is a major difference between genetic maps based on recombination frequency and physical…

A: Genetic map Genetic map is made from the recombinant frequency of genetic marker. Genetic map tells…

Q: Which crosses represent recombination in male gamete formation and which crosses represent…

A: Recombination frequency is used to measure the genetic linkage. It also used to create a genetic…

Q: 1.What is the genetic distance between C and D genes? a.22 CM b 2.2 cm c.220 kDa d.22 Mb…

A: During the meiosis stage of sexual reproduction, DNA sequences that are near together under a…

Q: . A haplotype is a specific set of SNPs and other genetic variants observed on a single chromosome…

A: DNA can be defined as the type of nucleic acid which is made from deoxyribose sugar along with four…

Q: 23. Which of the karyotypes shown is from a normal female? (LS3-1) * A 10 11 10 15 10 17 88 88 8 88…

A: Chromosomes are thread-like structures present inside the nucleus of the cell. They are made up of…

Q: typical STR DNA type originating from a single individual shows ________ peak(s) at a locus. a. one…

A: STR analysis is used as a tool in forensic analysis. It is used as a basis of variation between two…

Q: 13. If gene frequency between genes a and c is 3%; b and c is 15%; b and d 7%; a and b 18%; c and d…

A: Linkage is the tendency of nearby genes to remain close to each other and thereby, pass on to the…

Q: 8- Polymerase enzymes are used to a) Amplify the gene of interest b) Ligase vector and gene of…

A: Recombinant DNA or rDNA technology is a method used to join the DNA from two different species. The…

Q: Explain the V(D)J recombination of multiple gene elements

A: RECOMBINATION It is the process in which pieces of DNA are broken physically, exchanged, and then…

Q: 6. Section 2.6 describes several lines of support for the concept that "that variation in DNA on its…

A: A polymorphism is the multiple alternative forms within a protein or nucleic acid sequence. The…

Q: 3. Predict the number of DNA fragments and their sizes if Lambda phage DNA were incubated and…

A: Restriction enzymes are one of the important tools of recombinant DNA technology. The name…

Q: Item humbers 15 to 19. Refer to the figure below. (answers are in letters only) B A. B b a. B B A. a…

A: The exchange of genetic material between non-sister chromatids of homologous chromosomes is known as…

Q: Which of the following contain only the sequences required for transposition and the genes for…

A: Transposons are the DNA sequence which can change their position within the genome. It may be simple…

Q: 1) you have a locus in your PCR reaction that is a DNA squence is on the fifth chromosome, is a…

A: Gel electrophoresis is a common laboratory method used for the separation of DNA, RNA, or proteins…

Q: (AKS 8a2, DOK 1) Analyzing DNA with a DNA fingerprint developed by gel electrophoresis allows…

A: DNA fingerprint is a process that allows identifying paternity by compared among several…

Q: What is a contig?

A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…

Q: why does a clone of cells produceonly one type of G-6-PD enzyme? What would you expect to happen if…

A: Glucose-6-phosphate (G-6-PD) is the enzyme that is involved in the pentose phosphate pathway. They…

Q: 1. Identical twins may show dissimilar phenotypes due to changed methylation patterns of the…

A: Twins are two offspring produced by same mother in same pregnancy. Twins can be identical and non…

Q: In the Holliday model for homologous recombination shown, the resolution steps…

A: Deoxyribonucleic acid (DNA) is the hereditary material that undergoes replication which is vital for…

Q: 1. Drag the red box to indicate the restriction site in the following sequence. 5-.. АТА ТСА TСС TGT…

A: Genetic engineering is one of main principles of biotechnology. It is a process of manipulation of…

Q: 1. Give the Recombination Frequency of the following and show your solutions. a) Parents: AAbb x…

Q: 39 The structures labelled A and B in the image below represent: CTAAATCGGT Allele for red flowers B…

A: Answer: Incomplete Dominance : When the gene expression of one allele expresses completely and the…

Q: 34. A donor strain of bacteria with alleles a* b* c* is infected with phages to map the donor…

A: Transduction is a kind of horizontal gene transfer. There are found transfer of genes from the one…

Q: Make simple steps to extract isolating DNA from strawberries and yeast.

A: Answer

Question

2. Below is data from an HFR time of entry gene mapping experiment. Create a map of the
chromosome with all genes labeled and the location and orientations of the oriT sites.
Hfr Strain
Data
orit gal ton azi lac leu
orit azi ton gal trp cys
orit met ade1 arg cys trp
1
2
3
4
ortT met ade2 thr leu lac

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Similar questions

1. Choose the phrase from the right column that best fitsthe term in the left column.a. cytoplasmicsegregation1. transmission of genes through maternalgamete onlyb. heteroplasmic 2. cell that has mtDNAs or cpDNAs all of onegenotypec. homoplasmic 3. having gametes of similar sized. maternalinheritance4. a cell with a mixture of different mtDNAsgenerates a daughter cell with only one kinde. uniparentalinheritance5. a specific fraction of wild-type organellarDNAs is required for a wild-type phenotypef. isogamous 6. cell with mtDNAs or cpDNAs with differentgenotypesg. threshold effect 7. transmission of genes through either amaternal or a paternal gamete, but not both
1. _____________ frequencies are used to obtain a genetic map.A. TransductionB. ReconstitutionC. TransformationD. RecommendationE. Recombination 2. A __________ shows an individual cell's metaphase chromosomes arranged in pairs and sorted by size.A. phenotypeB. genotype C. karyotypeD. fingerprintE. none of the above 3. During conjugation, E.coli chromosomal transfer begins at ________.A. oriVB. oriCC. oriTD. oriAE. none of the above
2. What is the result of crossing over? A. recombination B. mutation C. linkage D. adaptation
Crosses are carried out between a bacterial F- strain and three different Hfr strains. In these strains, it takes 60 minutes for an Hfr strain to transfer the entire chromosome. Below are the data generated from interrupted mating experiments. Time of transfer of different genes (in minutes) is given for each strain. Appearance of genes in F- cells Hfr1: Genes b+ d+ c+ f+ g+ Time 3 5 16 27 59 Hfr2: Genes e+ f+ c+ d+ b+ Time 6 24 35 46 48 Hfr3 Genes d+ c+ f+ e+ g+ Time 4 15 26 44 58 Based on these data, you could conclude that Group of answer choices b is between g and d and is 4 minutes from g e is between f and g and is 25 minutes from g g is between e and b and is 24 minutes from e none of these is correct d is between b and c and is 10 minutes from b
6. A research group has selected three independent Trp−haploid strains of Neurospora, each of which cannotgrow in the absence of the amino acid tryptophan.They first mated these three strains with a wild-typestrain of opposite mating type, and then they analyzedthe resultant octads. For all three matings, two of thefour spore pairs in every octad could grow on minimalmedium (that is, in the absence of tryptophan), whilethe other two spore pairs were unable to grow on thisminimal medium.a. What can you conclude from this result?In the matings of mutant strains 1 and 2 with wild type,one of the two topmost pairs in some octads had sporesthat could grow on minimal medium while the other ofthe two topmost pairs in the same octads had spores thatcould not grow on minimal medium. In the mating ofmutant strain 3 with wild type, either all the spores inthe two topmost pairs could grow on minimal mediumor all could not grow on minimal medium.b. What can you conclude from this result?The…
Explain the 3 features of DNA markers.
2. Place this events in the correct order that they occur during homologous recombination: A. Resolvase cuts all four chromatidsB. Strand invasion causes one strand of the uncut chromatid to form a D loop C. Spo11 causes a double-strand break in a nonsister chromatidD. Branch migration lengthens the heteroduplex regionE. Recombinant chromosomes separate from each other
(1) Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring only total PE fluorescence? (2) Explain the effect that each mutation causes in the function of MC4R, and how they can be linked to disease.
1) Interpret the value of the interference. 2) Map the genes.
5. A haplotype is a specific set of SNPs and other genetic variants observed on a single chromosome or part of a chromosome, as compared between individuals in a population. Shown below are eight DNA sequences from different individuals: Nucleotide Position 1 5 10 15 Sequence 1. TCTGGAT CAT CACAT Sequence 2. ACAGCAT CATIA CGT Sequence 3. TCAGGAT CATIA CAT. Sequence 4. TCAGGAT CATI A CAT. Sequence 5. ACAGCATCATI ACGT Sequence 6. TCT G GAT CAT CACAT Sequence 7. TCAGGAT CATI A CAT. Luis Pagoada Sequence 8. ACAGCAT CAT IACGT. a. Give the nucleotide positions of all single nucleotide polymorphisms (SNPs; nucleotide positions where individuals vary in which base is present) in these sequences. b. How many different haplotypes (sets of linked variants) are found in these eight sequences?
Information obtained by microscopic analysis of stained chromosomes is used in: radiation hybrid mapping sequence mapping RFLP mapping cytogenetic mapping
Which of the following includes all of the others? a. homeotic genes b. master genes c. SRY gene d. PAX6