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How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?
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- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap please
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- At 10 am you received a doctors order that you have to Infuse 1,000ml of PNSS in 10 hours to Mr. Dela Cruz. What is the correct rate of this isotonic solution when the drop factor is 10 gtt = 1ml? 15.73 qtts/min- 31.249 qtts/min 16.67 qtts/min 18 qtts/minA plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?In the experiment performed for the determination of cholesterol, the function of the calibration curve obtained using 25-400mg/dL standard cholesterol solutions was determined as y= 0.0009x. If the absorbance of the serum sample is determined as 0.28, what is the amount of cholesterol in the sample, in mg/L?
- 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?The physician ordered: Digixin 250 mcg( micrograms) po qid. The label reads 1 tablet equals 0.25 mg(milligrams). How many tablets will you administer to your patient?If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not, what would it be?