What are the mechanism of action of hydrogen peroxide, rubbing alcohol, lab disinfectant and copper sulfate? Name a standard test for the effectiveness of a disinfectant. Name the chemical agent that is used as a standard. What are the standard test organisms?

Q: List two methods of high level disinfection.

A: The major difference between sterilization and disinfection is that sterilization involves the…

Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…

A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…

Q: What is a mixed culture? A contaminated culture?

A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…

Q: Match the test with the appropriate reagent/ chemical  a. DNA test b. Paper chromatography  c.…

A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…

Q: What is this test? What coated the bottom of the wells? What were we testing for? How did we know it…

A: The test is ELISA. It is an Enzyme linked Immunosorbant Assay, which is used in many applications.…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.

A: Protein is a nitrogenous organic macromolecule that is essential to human health. It is responsible…

Q: What is the media used in Catalase test and its expected results?

A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…

Q: explain why disinfectants that has a residual is preferable to one that does not

A: Introduction Disinfectants are chemical substances or compounds that are used to kill or inactivate…

Q: Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes…

A: * Disinfectant is a chemical substance that inactivates or to destroy the microorganisms that are…

Q: How would you identify this unknown bacteria using a flowchart and the bacteria below as a possible…

A: Biochemical tests in Microbiology help in distinguishing and identifying bacteria. Through the…

Q: What is the differece between disinfection and sterilization? What is the dfference between…

A: Disinfection and sterilization are essential for ensuring that medical and surgical instruments do…

Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…

A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…

Q: Make illustrated diagrams of stepwise procedures for the performance of the following tasks. 2.…

A: Microorganisms can be cultured in liquid medium or on solid medium. Culturing on solid medium…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.

A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…

Q: What is the importance of adding NSS and Iodine in stool specimen for smears? Compare the two

A: Stool examination is required any abnormalities in gastrointestinal functioning

Q: What are the different types of disinfectants? How is each one different or similar from the others?…

A: Almost every environment on the planet contains bacteria and microorganisms. In fact, on one square…

Q: Which medium is used for indole test and Coagulase Test?

A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…

Q: What are the examples of organisms that belong to the positive test in in-dose test?

A: Tryptophan is an amino acid that can undergo deamination. Indole is generated by reductive…

Q: Describe the Ninhydrin test. What does Ninhydrin test detect?

A: The ninhydrin test is a chemical procedure for detecting ammonia, primary/secondary amines, and…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: What is the difference between a Hemin test and a Benzidine test?

A: Hemoglobin is a blood protein that is responsible for oxygen transport in the tissues. Hemoglobin…

Q: What is the purpose of the sodium thiosulphate in the testing of treated water?

A: Testing of wastewater and treated water both demand a standardized process to be followed to obtain…

Q: Discuss the significance of the following processes and what constituent is being determined: a.…

A: Borntrager’s Test: This is a quantitative chemical test for the glycosides in any given sample, this…

Q: Why are most antimicrobial chemical agents disinfectants rather than sterilants? What general…

A: Most chemical antimicrobial agents are disinfectants instead of sterilants. A disinfectant reduces…

Q: What is the media used in Urease Test and expected results?

A: Urease test is defined as a biochemical test that distinguishes the basic alkaline fermentation of…

Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.

A:

Q: What is the principlels of in-dose test?

A: During the development of drugs, the efficacy of a new drug is typically determined by showing its…

Q: Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the…

A: In the laboratory blood samples are used to detect various disease conditions. There are different…

Q: What is the most commonly used fixative in stool samples and why?

A: Fixative is a moist environment for transporting and preserving intestinal parasites in stool…

Q: List one method of disinfection, and one method of sterilization. Label which is which.

A: Disinfection is a process by which most of the harmful microbes are killed or inactivated. However…

Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?

A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…

Q: Differentiate therapeutic sterilization from direct sterilization What are some of the…

A: Sterilization is a medical procedure where the reproductive ability of an individual is taken off.…

Q: What special precautions must be taken when using the oil immersion (100x) objective?

A: Using oil immersion reduces the air gap between the glass slide and the objective lens. This air gap…

Q: What items are considered semi-critical and what is their level of disinfection in the Operating…

A: A hospital is a place or health care institution that provides care and treatment to the patients…

Q: Why do we add powdered zinc to the nitrate reduction test?

A: Bacteria are prokaryotic simple unicellular organisms. Their mode of respiration may be aerobic or…

Q: what is the purpose and objectives on doing nitrious acid test?

A: Amines are the compounds and functional groups having a basic nitrogen atom with a lone pair of…

Q: How can organisms develop resistance to chemical disinfectants?

A: Bacteria can develop resistance to disinfectants over time either by acquisition of exogenous mobile…

Q: Define the following terms; Sterilization, and Aseptic Technique. What are the differences between…

A: Microbes invading and proliferating within an organism's body causes infection. Microbes include…

Q: Other than the PYR test, what is another use of the PEP reagent?

A: PYR is a colorimetric method used to detect the presence of pyrolidonyl arylamidase activity in a…

Q: Identify the principles and procedures related to disinfection and sterilization.

A: Sterilization is the process of killing microorganisms along with their spores. It is the most…

Q: which kind of sample gives positive result for Molish test?

A: It is a chemical test which is named after the botanist Hans Molisch.  This test involves the…

Q: In the conduct of serial dilution, which of the following tools is NOT necessary?   a. Agar…

A:

Q: What are the immersion media that need to be used?

A: Immersion media is used to increase the numerical aperture of the microscope by a factor equal to…

Q: What method can be used to achieve sterilization?

A: It is required to describe the method to be used to achieve sterilization.

Q: what practical importance are air borne microorganisms to the laboratory workers?  What precautions…

A: Laboratories are the areas where lot of experiments and research is performed. It deals with lot of…

Physical and Chemical features

The physical property of a compound could be determined without modifying the originality of the compound. Examples of physical properties are density, hardness, color, boiling, and melting point. The chemical property expresses the ability of a compound to undergo a particular chemical change.