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- Activity 1.4: Essay.Direction: Explain your answer.Today, it is easy to make transgenic plants and animals. What are some important safety and ethical issues raised by this use of recombinant DNA technology? Explain your answer.#2. Solution needed How might we, as epidemiologists, increase the validity of genetic studies? What recommendations do you have (assume that you have all of the resources you'd need to implement these changes)?.How does SYBR green work in DNA imaging and why does the uncut plasmid run faster than the cut plasmid? Please Explain. Thank you
- Describe the main technique for amplifying a segment of DNA (like the one you suspect is involved in Lee’s cancer) from a complex mixture of genomic DNA. Remember that the entire human genome sequence is known. (Hint: This is a technique that is commonly used by laboratories that do genetic testing and various other applications of molecular biology.)Topic: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting factors) Question When or why is this genetic technology/process used? Who benefits from this genetic technology/process - and how?Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein) of different sizes and topological forms. (Include in you answer the different recombinant DNA techniques that require gel electrophoresis)
- Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation. Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319Expand PCR? Describe the different Steps involved in this technique?After properly troubleshooting, you finally are able to visualize the gel. You obtained the image below. The ladder used is “Lambda Hind III”. 1. Looking at the gel results, what can you say about the quality of DNA extraction for protocols 1, 2 & 3? 2. What would you take into consideration when selecting a molecular marker or leader to include in your gel run?
- a.) Explain briefly how silica allows us to purify DNA. Be sure to state the relevant biochemical features of silica and DNA as well as any relevant reagents or steps that are important for the purification. Your answer should explain both how we get the DNA to bind in the first place, and how we are able to remove the DNA. You do not (and should not) explain earlier steps in a DNA purification; focus you answer on the silica:DNA interaction. b.) In theory, can a silica spin column also be used to purify RNA? If so, explain how (and why we did not observe RNA when analyzing plasmid DNA via agarose gel electrophoresis). If not, explain why not.SequencingIn order to ensure a reliable result, arrange the following procedure with A being the first and F the last. A. Place each onion on the glasses filled with Distilled water, with 50% Substance A and 50% distilled water, with 50% Substance B and 50% distilled water. The base of the onions must be submerged in the solution. B. Record your data and analyze. C. Place the set-ups in a well-lighted area for 2-5 days. D. If the onion is too small for the glass, poke four toothpicks, equally spaced, around the circumference of the mid portion of the onion for support. E. Choose onion bulbs without shoots. Cut the roots of the onion bulb, making sure that there are no new or old roots present. F. Count the number of roots and shoots that has grown and measure their length.SequencingIn order to ensure a reliable result, arrange the following procedure with A being the first and F the last. A. Place each onion on the glasses filled with Distilled water, with 50% Substance A and 50% distilled water, with 50% Substance B and 50% distilled water. The base of the onions must be submerged in the solution. B. Record your data and analyze. C. Place the set-ups in a well-lighted area for 2-5 days. D. If the onion is too small for the glass, poke four toothpicks, equally spaced, around the circumference of the mid portion of the onion for support. E. Choose onion bulbs without shoots. Cut the roots of the onion bulb, making sure that there are no new or old roots present. F. Count the number of roots and shoots that has grown and measure their length.SequencingIn order to ensure a reliable result, arrange the following procedure with A being the first and F the last. A. Place…