Design a strategy to make a subtracted-complete cDNA library from liver cells. Use a flowchart method to propose how this objective can be accomplished. The sizes of mRNA range from 2 kb to 15 kb. Briefly indicate your reasons for the sequence of steps..
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Design a strategy to make a subtracted-complete cDNA library from liver cells. Use a flowchart method to propose how this objective can be accomplished. The sizes of mRNA range from 2 kb to 15 kb. Briefly indicate your reasons for the sequence of steps..
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- What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.Refer to the figure. What method would you use if you wanted to determine the sequence of the cDNA of the peak protein? Briefly explain the sequencing methoda molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. he knows that the nucleotide sequence of a small part of the gene is gtggactgaca. briefly explain how to obtain the desired gene answer
- Discuss the following statement: “from the nucleotide sequence of a cDNA clone, the complete amino acid sequence of a protein can be deduced by applying the genetic code. thus, protein biochemistry has become superfluous because there is nothing more that can be learned by studying the protein.”Analyzing Cloned Sequences What kind of information can a DNA sequence provide to a researcher studying a disease-causing gene?Give typing answer with explanation and conclusion Suppose that RNA polymerase was transcribing a eukaryotic gene with several introns all contained within the coding region. In what order would the RNA polymerase encounter the elements in the DNA sequence of the gene? Earliest encountered promoter 5' UTR translation initiation codon splice branch point stop codon 3' UTR Latest encountered
- After Create a second cDNA strand complementary to the first. After the reaction is completed, the enzyme S1 nuclease is used to cleave the hairpin loop., what is the final step of making a cDNA library?True or False; explain It is possible that the coding region of a gene is present in a genomic library produced from a particular tissue, but is absent in cDNA library prepared from the same tissue.How does polio (poliovirus) get more than one protein from a single mRNA? Give typing answer with explanation and conclusion
- A molecular geneticist hopes to find a Gene in human liver cell that codes for an important blood-clotting protein,he knows that the nucleotide sequence of a small part of the Gene is GTGGACTGACA.briefly explain how to obtain geneRefer to the DNA sequence provided:3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’a. What is the mRNA transcript of the anticoding strand of the DNA model?Could quantitative PCR, which uses a DNA-binding dye, to show how many copies of the target DNA sequence could be used to quantify the amount of mRNA in a cell? Would you expect that a metabolically active tissue such as the liver would show more cDNA copies in such a method, compared to less metabolically active tissues such as skin cells? One reason that the types and amounts of mRNAs are quantified in different tissue types is to compare which genes are activated and which are inactive. It used to be thought that any gene that was transcribed was automatically translated. The discovery of RNA-degrading systems shows that the real situation in cells is more complemented. Do you believe that a larger amount of mRNA of a given type, say for alpha hemoglobin in immature red blood cells is a reliable indicator that more alpha hemoglobin protein will be made in those cells?