di-deoxy nucleotides terminate DNA elongation in Maxam-gilbert method. True False
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- A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than one with 2,500 bp.True or false?Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two complementary DNA fragments. DiscussWe use 3.16 fold dilution why this dilution schedule is important to determine unknown DNA concentration?
- How is the melting curve of duplex DNA aff ected by adding a small amount of ethanol?Why is DNA not soluble in ethanol whereas other components cells are soluble. What is the rationale behind DNA precipitation in solutionWhat is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive charge, and as such is not soluble in alcohol, and therefore precipitates. DNA is a non polar molecule with an overall negative change, and as such is soluble in alcohol, and therefore precipitates. DNA is a polar molecule with an overall positive change, and as such is soluble in alcohol, and therefore precipitates.
- Sliding-clamp proteins load polymerase onto the primer and maintain its stable association with the template. true or false? DNA polymerases add a deoxyribonucleotide triphosphate to the 3' hydroxyl group of a growing DNA chain (the primer strand). true or false?A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2% agarose gel instead, what would you expect would happen to the migration rate of DNA and why?#1: 3’ T A C A T G C C G A A T G C C 5’ #2: 3' T A C T G G C A T A A C A C T 5' determine the following Partner DNA strand - the mRNA strand - the tRNA - the formed amino acids- the discussion of the entire procedure -
- proteins are denatured and eliminated during the DNA extraction process. how can we determine the purity of our DNA sample?Which of the following substances adds equivalent DNA nucleotides to the 3' end of Okazaki fragments after primers are removed? Group of answer choices helicase DNA ligase DNA polymerase I DNA polymerase IIIExplain how an agarose gel can separate DNA fragments of different lengths.
