Digestion by EcoRI Digestion by Smal Digestion by both EcoR alone 3-kb fragment 5-kb fragment 6-kb fragment alone and Smal 2-kb fragment 3-kb fragment 4-kb fragment 5-kb fragment 7-kb fragment 7-kb fragment

Body Structures & Functions
12th Edition
ISBN:9781285695495
Author:Scott
Publisher:Scott
Chapter18: Digestive System
Section: Chapter Questions
Problem 2TOF
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A linear piece of DNA that is 14 kb long is cut first by EcoRI alone, then by SmaI alone, and finally, by both EcoRI and SmaI together. The following results are obtained:

Draw a map of the EcoRI and SmaI restriction sites on this 14-kb piece of DNA, indicating the relative positions of the restriction sites and the distances between them.

Digestion by EcoRI Digestion by Smal Digestion by both EcoR
alone
3-kb fragment
5-kb fragment
6-kb fragment
alone
and Smal
2-kb fragment
3-kb fragment
4-kb fragment
5-kb fragment
7-kb fragment
7-kb fragment
Transcribed Image Text:Digestion by EcoRI Digestion by Smal Digestion by both EcoR alone 3-kb fragment 5-kb fragment 6-kb fragment alone and Smal 2-kb fragment 3-kb fragment 4-kb fragment 5-kb fragment 7-kb fragment 7-kb fragment
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