With the principles of GFC, which of the following statements is incorrect? a. For small molecules, their elution volume, Ve > VO b. For a very large molecule, its elution volume, Ve = Vo c. Blue dextran is used to determine the void volume because it is too large and cannot get into the beads and therefore excluded from the gel. d. Low molecular weight molecules are freely accessible to the gel particles, and elute at a volume equal to the internal pore volume. In an SDS-PAGE experiment, protein X has an Rf = 0.75, while protein Y has an Rf = 0.30. Which of the following statements are FALSE during the electrophoretic run? I. X has a greater charge-to-mass ratio than Y, since X has a smaller MW. II. X has a lower MW, and thus experiences less friction with the gel matrix. III. X has more hydrophobic groups, thus is in a more linearized state. IV. X is globular, while protein Y is rod-like, thus X has higher mobility. V. X is affected by less magnitude of electric field because of smaller MW. a. I and V b. II and IV c. II, III, and IV d. I, III, IV, and V
With the principles of GFC, which of the following statements is incorrect?
a. For small molecules, their elution volume, Ve > VO
b. For a very large molecule, its elution volume, Ve = Vo
c. Blue dextran is used to determine the void volume because it is too large
and cannot get into the beads and therefore excluded from the gel.
d. Low molecular weight molecules are freely accessible to the gel particles,
and elute at a volume equal to the internal pore volume.
In an SDS-PAGE experiment, protein X has an Rf = 0.75, while protein Y has an Rf
= 0.30. Which of the following statements are FALSE during the electrophoretic
run?
I. X has a greater charge-to-mass ratio than Y, since X has a smaller MW.
II. X has a lower MW, and thus experiences less friction with the gel matrix.
III. X has more hydrophobic groups, thus is in a more linearized state.
IV. X is globular, while protein Y is rod-like, thus X has higher mobility.
V. X is affected by less magnitude of electric field because of smaller MW.
a. I and V
b. II and IV
c. II, III, and IV
d. I, III, IV, and V
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