With the aid of a diagram, explain the principles of gel filtration chromatography.
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With the aid of a diagram, explain the principles of gel filtration chromatography.
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- With the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGEBriefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatographyExplain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.
- Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.How does gel filtration work? What are the applications of analytical versus preparative versions of the method ?Describe in detail the process of electrospray ionization ?
- CREATE A SCHEMATIC DIAGRAM OF THE ASSAY OF SODIUM CHLORIDEState the functions for each of these components used in the gel filtration chromatography using a column experiment. Bromophenol blue A mixture of albumin and bromophenol blue SpectrophotometerIf you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagram