Ideal solvents for fat extraction should: a- penetrate sample particles readily, be in single component form to achieve fractionation b- penetrate sample particles readily, be in single component form to avoid fractionation c- be hygroscopic d- be non-hygroscopic e- a+ c f- b+ d O g a+d h-b + c
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- An average protein will not be denatured by: a. a detergent such as sodium dodecyl sulfate. b. heating to 90°C. c. sodium chloride d. pH 10. e. urea.The 3 proteins have the following properties. Which is the correct order of steps to extract them? + ethyl acetate, + (NH4)2SO4 at 20% saturation, centrifugation, + detergent (Triton X), buffer the lysate (pH=10), centrifugation, collect precipitate + ethyl acetate, + detergent (Triton X), + (NH4)2SO4 at 20% saturation, centrifugation, buffer the lysate (pH=10), centrifugation, collect precipitate + 1 M NaCl, + detergent (sodium dodecyI suIfate), centrifugation, +(NH4)2SO4 at 60% saturation, buffer at pH 7, centrifugation, collect precipitate + ethyl acetate, adjust pH to 7.3, centrifugation, collect supernate, +detergent (Triton X) to precipitate, +(NH4)2SO4 at 30% saturation, centrifugation, collect precipitateIf there are still contaminating proteins with your LDH fraction after going through 2 different purification schemes (affinity and ion exchange chromatography), what would be their physical characteristics
- During extractions, it is common to add a dessicant/drying agent (such as magnesium sulfate) to absorb trace amounts of water from the organic layer (e.g., dichloromethane). If you were adding magnesium sulfate to 50 mL of dichloromethane in a 100 mL flask, how much MgSO4 should you add? 1.0 g 0.5 g the smallest amount possible (just enough to be visible) enough to fill the flask halfway the correct amount is determined by observation. MgSO4 is added until the drying agent no longer clumps and there is no visible water floating on the surfaceIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperYour supervisor asks to make 350 mL of a 0.75% (w/v) starch solution using a 5% (w/v) stock solution. You have calcuated how much stock solution you need. But, how much water (in mL) will you need to add to the stock to achieve the correct final concentration?
- Which of the following is the best explanation for a gel like this?A. Bromophenol blue was not added in the loading buffer.B. Glycine was not present in the buffer system.C. There was too much bisacrylamide in the gel solution.D. Gel was not overlaid after pouring the first gel solution.1. Calamine Lotion is classified as a suspension. What is a suspension? A. It is a disperse system in which solid, vehicle-insoluble particles (internal phase) are uniformlysuspended by mechanical agitation and formulation design throughout the liquid vehicle(external phase).B. It is a clear, flavored, sweetened, hydroalcoholic liquid oral preparation that usually containeither potent or unpleasant tasting drugsC. It is a concentrated aqueous solution containing one or more sugar components, chiefly sucrose,or sugar substituteD. It is a viscous oral liquid containing one or more medicament dissolved in a vehicle that usuallycontains high proportion of sucrose or other sugars.A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, and phosphatidylcholine was applied to a hydrophobic interaction chromatography column. The column was then washed with increasingly nonpolar solvents. IWhat order would the lipids be eluted from the column?
- Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a blue solution and the recipe for 2X Laemmli buffer is: 0.125 M Tris HCl, pH 6.8, 20% glycerol, 4% SDS and 10% β-mercaptoethanol (βME). What is the purpose of each of these ingredients?A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.