Recombinant DNA Cloning DNA sequencing Polymerase chain reaction Purpose: 14. Purpose: 15. Purpose: to produce very large numbers of identical DNA molecules

Q: DNA Template Parental DNA New DNA THINK THIS OVER: 1. How can you relate this experiment in…

A: This experiment shows replication according to the conventional theories. According to this…

Q: Which activity of E. coli DNA polymerase I is responsible for proofreading the newly synthesized…

A: The first known polymerase is the DNA Polymerase I. It is the enzyme that participates in…

Q: Polymerase chain reaction (PCR) is a technique used to amplify a fragment of DNA. When conducting…

A: PCR is a method used to rapidly make millions of copies of a specific DNA sample and amplify DNA…

Q: 2- Supposed that you have a DNA sequence sample with 10,000 bp. By using 2 different restriction…

A: The answer is 3.

Q: DNA template A complementary DNA primer Single-stranded RNA template Four deoxynucleotides (DATP,…

A: Introduction:- The process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…

Q: explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain…

A: PCR is the polymerase chain reaction that use to amplify the particular sequence of DNA and forming…

Q: Please compare and contrast these three OR make table: 1.qPCR 2. RT-PCR 3. PCR

A: Polymerase chain reaction (PCR) is a scientific instrument that can be used to make a large number…

Q: 1.) Describe the components that are important to a cloning vector.

A: The term vector refers to the DNA molecules that act as transporting vehicle which carries foreign…

Q: The way that PCR amplifi es DNA is similar to the doubling in a population of growing bacteria; a…

A: A molecular biology technique that is performed to amplify the deoxyribonucleic acid (DNA) molecules…

Q: 9. Why does the lagging strand need Ligase? * O Polymerase runs 5' to 3'. This creates fragments and…

A: Replication is the process which is responsible for producing daughter DNA molecules from the…

Q: Give 2 advantages of SPACER DNA SEQUENCES as regions in the genome/chromosome to target for DNA…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: What did some think about Watson and Crick that they would not be productive

A: DNA has a double-helix structure, with sugar and phosphate on the outside of the helix, forming the…

Q: DNA cloning isa. making multiple genetically identical cells.b. making multiple copies of a piece of…

A: Molecular biology deals with the molecular basis of the cells including the synthesis,…

Q: Copying errors that slip by DNA polymerase proofreading can be corrected by DNA ligase. direct…

A: The major inheritable material is the Deoxyribonucleic Acid (DNA). The mechanism of copying…

Q: 2. Which of these statements is INCORRECT about restriction enzymes Restriction enzymes form…

A: Restriction enzymes originated from bacteria

Q: What goes into the reaction tube for Sanger sequencing?   Template DNA, primers, DNA polymerase,…

A: Sanger sequencing was developed by  Frederick Sanger and his colleagues in the year 1977 to…

Q: 30.Match the definition with one of the choices on the right (can be used more than once) Place with…

A: DNA or deoxyribonucleic acid is the main molecule that is responsible for the purpose of storing…

Q: Double-stranded DNA replicates in a _____manner. When __________ strands separate, each serves as…

A: DNA (deoxyribonucleic acid) is a long polymer of deoxyribonucleotides. Chemicall. DNA is composed of…

Q: Create a 75-nitrogenous base long DNA and replicate it completely. Make sure initiation, elongation…

A: Introduction The process of replicating a double-stranded DNA molecule into two identical DNA…

Q: 2. PCR analysis practice One 1) If you collect samples from crime scene and extract DNA out of each…

A: For the amount of polymorphism in repeated sequences, any part of the DNA sample that is found in a…

Q: 1. You are a research assistant in a renowned science academy. Your supervisor requires you to…

A: PCR:- it is used to amplify the fragments of a DNA or gene of interest.  Gene cloning:- it is a…

Q: Your colleague made the table below comparing DNA Polymerase to RNA Polymerases. Which row is…

A: Transcription is the molecular process which involves the DNA sequences that are transcribed as…

Q: 8- Briefly state why heat-stable DNA polymerase from a thermophilic bacterium is used in PCR instead…

A: Polymerase chain reaction is the method of multiplying an specific sequence of dna to obtain it's…

Q: 1) Follow the steps involved in DNA or gene cloning

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: DNA polymerase III adds nucleotides to both ends of the RNA primer to the 5' end of the RNA primer

A: DNA is the genetic element found in all prokaryotic and eukaryotic cell types. DNA is a…

Q: DNA polymerase III adds a nucleotide to the 3' end of the strand. leading strand. All of the answers…

A: Concept used:  DNA Replication DNA Replication : The process where the strands of DNA are…

Q: What enzyme proofreads the DNA molecule?   Group of answer choices DNA gyrase DNA polymerase I DNA…

A: DNA replication is the process of synthesis of a new DNA molecule from a parent DNA by the enzyme…

Q: PCR is quick, efficient and easy to perform. However, there are some situations when cell-based…

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: DNA Polymerase Another enzyme, and arguably the most important, is DNA polymerase. This enzyme's…

A: DNA Polymerase and DNA Primase are the Enzymes responsible for the DNA replication process.

Q: DNA polymerases cannot initiate synthesis of a polynucleotide; they can only add nucleotides to the…

A: DNA is the genetic material in organisms.  It is formed via polymerization of nitrogen base ,…

Q: Polymerase chain reaction (PCR) is a technique used to amplify a fragment of DNA. When conducting…

A: PCR or Polymerase Chain Reaction is the procedure of amplifying a DNA segment. It was invented by…

Q: MOLECULAR CLONING • Molecular cloning is the laboratory process used to create recombinant DNA. • It…

A: Recombinant DNA: it is a method of combining two distinct DNA (molecule) sequences, which may or may…

Q: scientist uses a molecule of DNA composed of radioactive nucleotides as a template for replication…

A: DNA replication is the process of formation of exact duplicate copy of DNA. Transcription RNA is…

Q: #2 under Process: DNA is copied from the 3' prime to the '5 prime end.   True   False

A: DNA replication is the semiconservative process of formation of the daughter DNA strand from the…

Q: cDNA, a term used in recombinant DNA technology meansa) Competitive DNAb) Chemical DNAc) Complex…

A: Deoxyribonucleic acid (DNA) is the genetic material of most organisms. DNA contains the instructions…

Q: In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C…

A: PCR (polymerase chain reaction) is a technique for analysing a short sequence of DNA (or RNA) also…

Q: Sequencing Number the steps of DNA replication in the correct order 1 being the first & 5 for the…

A: DNA is the genetic material .DNA  replication  ensure that new cell is receiving the correct number…

Q: 2. Compare the key players in DNA replication, PCR, transcription, and translation by filling out a…

A: The process of creating two identical copies of DNA from the original DNA is known as DNA…

Q: During the second step of PCR, the temperature is lowered in order to: denature the taq polymerase…

A: The term 'PCR' stands for Polymerase chain reaction wherein a single DNA fragment can be used as a…

Q: Create a 75-nitrogenous base long DNA and replicate it completely. Make sure initiation, elongation…

A: Replication of DNA :  DNA replication is the process where a new strand exactly similar to the…

Q: 3- Which of the following is not a basic requirement of PCR? a-Taq DNA polymerase b-DNA Probes…

A: Polymerase Chain Reaction (PCR). Principle: When a double-stranded DNA molecule is heated to a high…

Q: A scientist adds a chemical to a culture of dividing cells in order to disrupt DNA replication. The…

A: DNA replication is a very important process of the central dogma. The process involves many enzymes…

Q: 1. What is DNA cloning of animals and plants? 2. What is the importance of DNA cloning?  3. Give…

A: 1. DNA cloning of animals and plants is a process of creating a genetically identical copy of an…

Q: RECOMBINANT DNA • Recombinant DNA molecules are sometimes called chimeric DNA. • The DNA sequences…

A: Recombinant DNA technology is very commonly used in laboratories that is responsible for the…

Q: An enzyme to add nucleotides specifically at the ends of chromosomes would be: Question 6 options:…

A: Chromosomes are the carrier of hereditary DNA molecules and have a thread-like appearance. They are…

Q: A biochemist isolates, purifies and combines ina test tube a variety of molecules needed for DNA…

A: According to the central dogma of molecular biology, DNA is converted to RNA (transcription) and RNA…

Q: If you need to make a lot up to 1 billion copies of a piece of DNA you would use 1. gel…

A: Polymerase chain reaction is the method of making millions of copies of a specific DNA.  It was…

Q: Which of the following best describes the complete sequence of steps occurring during eve cycle of…

A: PCR is an abbreviation for polymerase chain reaction which was developed by the biochemist Kary…

Environmental health and hazards

An organism's environment is the environment in which it lives and has an impact on its well-being. The World Health Organization (WHO) defines environmental health as "those aspects of public health, such as quality of life, that are influenced by physical, chemical, biological, social, and psychosocial components in the environment." Environmental health is concerned with both natural and man-made settings in order to enhance public health. Environmental science, occupational medicine, toxicology, and epidemiology are the key subdisciplines of environmental health.