Using a Sep-Pak C18 column(Vm = 0.49 mL) in order to separate 2 dyes from an ink sample. The two dyes, Dye 1 and Dye 2, were eluted from the column with a constant flow of 18% isopropanol. Dye 1Dye 2Run 1Run 2Run 3Run 1Run 2Run 3Start of Band (mL)1.11.11.22.22.22.3End of Band (mL)1.81.71.83.94.14.2Solve for the:Retention Volume of Dye 1 and 2Capacity Factor of Dye 1 and 2Separation FactorColumn Resolution

Retention Volume : It is the volume of the mobile phase which is required to elute out a particular…

Dye 1 Dye 2 Run 1 Run 2 Run 3 Run 1 Run 2 Run 3 Start of Band (mL} 1.1 1.1 1.2 2.2 2.2 2.3 End of Band (mL) 1.8 1.7 1.8 3.9 4.1 4.2 Solve for the: Retention Volume of Dye 1 and 2 Capacity Factor of Dye 1 and 2 Separation Factor Column Resolution

Dye 1 Dye 2 Run 1 Run 2 Run 3 Run 1 Run 2 Run 3 Start of Band (mL} 1.1 1.1 1.2 2.2 2.2 2.3 End of Band (mL) 1.8 1.7 1.8 3.9 4.1 4.2 Solve for the: Retention Volume of Dye 1 and 2 Capacity Factor of Dye 1 and 2 Separation Factor Column Resolution

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 13.16QAP

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Concept explainers

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

Using a Sep-Pak C18 column(Vm = 0.49 mL) in order to separate 2 dyes from an ink sample. The two dyes, Dye 1 and Dye 2, were eluted from the column with a constant flow of 18% isopropanol.

Dye 1
Dye 2
Run 1
Run 2
Run 3
Run 1
Run 2
Run 3
Start of Band (mL)
1.1
1.1
1.2
2.2
2.2
2.3
End of Band (mL)
1.8
1.7
1.8
3.9
4.1
4.2
Solve for the:
Retention Volume of Dye 1 and 2
Capacity Factor of Dye 1 and 2
Separation Factor
Column Resolution

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